1、Designation:E104500(Reapproved 2010)Standard Specification forPipet,Sahli Hemoglobin1This standard is issued under the fixed designation E1045;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in pare
2、ntheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification covers reusable pipets calibrated“tocontain”20 cmm of whole blood and used for hemoglobindeterminations.1.2 The values stated in SI un
3、its are to be regarded asstandard.No other units of measurement are included in thisstandard.2.Referenced Documents2.1 ASTM Standards:2E920 Specification for Commercially Packaged LaboratoryApparatusE921 Specification for Export Packaged Laboratory Appa-ratusE1133 Practice for Performance Testing of
4、 Packaged Labo-ratory Apparatus for United States Government Procure-mentsE1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3.Materials3.1 The pipets shall be made of common spirit bore whiteback tubing or of clear glass with a white stripe applied to theouter surface of th
5、e tubing.4.Design4.1 ShapePipets shall be straight and of one-piece con-struction.Any cross section of a pipet taken in a planeperpendicular to the longitudinal axis should be circular.4.2 Delivery TipsDelivery tips shall be made with agradual or concave taper to a length of 10 to 25 mm.The tipend s
6、hall be ground and tapered with fine abrasive,or fire-polished.Dimensions of the delivery tip shall be as specified inFig.1.4.3 The top of the pipet shall be ground to a taper or formedto a funnel shape according to the dimensions specified in Fig.1.5.Markings5.1 Graduation LineThe pipet shall have
7、one graduationline located 20 cmm from the pipet tip.The graduation lineshall be located on the clear portion of the tubing and shallextend at least two thirds around the pipet and not exceed 0.4mm in width.5.2 Volumetric DesignationThe pipet shall be marked20CMM on the clear portion of the tubing w
8、ith the markingslocated approximately 5 mm above the graduation line.5.3 IdentificationEach pipet shall be marked with themanufacturers name or trademark on the white stripe portionof the pipet.Catalog number markings are optional.Allmarkings shall be permanently fused onto the pipet.Themarkings sha
9、ll be amber or black in color.When tested inaccordance to 6.3,the pigmentation shall not discolor.Theappearance of the markings,when viewed by the eye undernormal room lighting,shall be the same before and aftertesting.5.4 Capacity DeviationSahli Hemoglobin pipets are madewith maximum capacity devia
10、tion of 61.0%or 62.0%.Theselected capacity deviation shall be marked on the clear orwhite stripe portion of the pipet.The capacity of the pipet shallbe within the selected capacity deviation marked on the pipetwhen tested as specified in 6.2.6.Testing6.1 Capacity TestThe capacity of the pipet shall
11、bedetermined by means of using distilled water and a weighingdevice with weight sensitivity not less than 0.001 mg.6.1.1 The pipet shall be thoroughly cleaned,dried,andallowed to adjust to room temperature.6.1.2 The pipet shall be weighed and the weight recorded.6.1.3 The pipet shall be filled to th
12、e calibration line withdistilled water and weighed,and the weight recorded.6.1.4 The recorded weight of the clean and dry pipet shall besubtracted from the recorded weight of the distilled water-filledpipet providing the observed volumetric capacity(Vc)of thepipet in grams.1This specification is und
13、er the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved July 1,2010.Published July 2010.Originally approvedin 1985.Last previous edition approved in 2005 as E1045 00(2005).DOI:10.1520/E1045-00R10.2F
14、or referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West
15、Conshohocken,PA 19428-2959.United States1 6.1.5 The observed volumetric capacity(Vt)shall then becorrected to actual volumetric capacity at 20C(Vc),deter-mined by:Vc5Vt11at 2 20C!(1)where:Vt=observed volumetric capacity at t C,grams,Vc=corrected volumetric capacity at 20C,a=coefficientofcubicalexpan
16、sionofpipetglass,=0.000072 C 0.000084 C(dependent uponsource of material),andt=temperature recorded during weighing,C.6.2 Capacity DeviationThe capacity deviation of the pipetis the difference between the stated capacity V1and thecorrected observed capacity Vc,and is determined by thefollowing expression:Capacity deviation,%5100Vc2 V1!V1(2)6.3 Pigmentation TestPrepare a fresh chromic acid clean-ing solution by combining 200 g of solution dichromate(Na2Cr2O72H2O),1000 mLof water,and 1500 mLof sul