1、Designation:E115314Standard Test Method forEfficacy of Sanitizers Recommended for Inanimate,Hard,Nonporous Non-Food Contact Surfaces1This standard is issued under the fixed designation E1153;the number immediately following the designation indicates the year oforiginal adoption or,in the case of rev
2、ision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is used to evaluate the antimicrobialefficacy of sanitizers on precleaned,inanimate,hard
3、,nonporous,non-food contact surfaces against Staphylococcusaureus,or Klebsiella pneumoniae or Enterobacter aerogenes,or a combination thereof.Appropriate modifications to themethod may be required when testing organisms not specifiedherein.When utilizing test surfaces not described herein(seeTest Me
4、thod E2274)or when evaluating spray-based ortowelette-based antimicrobial products,modifications may alsobe required.1.2 This test method may also be used to evaluate theantimicrobial efficacy of one-step cleaner-sanitizer formula-tions recommended for use on lightly soiled,inanimate,nonporous,non-f
5、ood contact surfaces.1.3 It is the responsibility of the investigator to determinewhether Good Laboratory Practices(GLP)are required and tofollow them where appropriate(see section 40 CFR,160 or asrevised.)1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement
6、are included in thisstandard.1.5 This standard may involve hazardous materials,chemi-cals and microorganisms and should be performed only bypersons who have had formal microbiological training.Thisstandard does not purport to address all of the safety concerns,if any,associated with its use.It is th
7、e responsibility of the userof this standard to establish appropriate safety and healthpractices and determine the applicability of regulatory limita-tions prior to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE1054 Test Methods for Evaluation of Inactivators of
8、 Anti-microbial AgentsE2274 Test Method for Evaluation of Laundry Sanitizersand DisinfectantsE2756 Terminology Relating to Antimicrobial and AntiviralAgents2.2 Federal Standard:40 CFR,Part 160,Good Laboratory Practice Standards33.Terminology3.1 Terms used in this test method are defined in Terminol-
9、ogy E2756.3.2 Definitions of Terms Specific to This Standard:3.2.1 accuracy,na measure of the degree of conformity ofa value generated by a specific procedure to the assumed oraccepted true value,and includes both precision and bias.3.2.2 ambient temperature,ntemperature of the environ-ment in which
10、 a test method is performed.3.2.3 antimicrobial,adjdescribes an agent that kills orinactivates microorganisms or suppresses their growth orreproduction.3.2.4 bias,na systematic error that contributes to thedifference between the mean of a large number of test resultsand an accepted reference value.3
11、.2.5 cleaner-sanitizer,na physical or chemical agent thatremoves soil from an object and reduces numbers of microor-ganisms on non-food contact surfaces.1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directrespo
12、nsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved April 1,2014.Published May 2014.Originallyapproved 1987.Last previous edition approved in 2010 as E1153 03(2010)1.DOI:10.1520/E1153-14.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM C
13、ustomer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from the Superintendent of Documents,U.S.Government PrintingOffice,Washington,DC 20402.Copyright ASTM International,100 Barr Harbor Drive,
14、PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.2.6 carrier,na surrogate surface or matrix that facili-tates the interaction of test microorganisms and treatment(s).3.2.7 effcacy,nthe proven performance of a productestablished under defined conditions of testing.3.2.8 inoculum,nthe viab
15、le microorganisms used to con-taminate a sample,device or surface,often expressed as tonumber and type.3.2.9 neutralization,nthe process for inactivating orquenching the activity of a microbiocide,often achievedthrough physical(for example,filtration or dilution)or chemi-cal means.3.2.10 precision,n
16、the closeness of agreement betweenindependent test results obtained under prescribed conditions.3.2.11 reproducibility,nthe precision of test results ob-tained in different laboratories performing the same test pro-cedure under specifically defined conditions.3.2.12 sanitizer,nchemical or physical agent(s)used toreduce the number of microorganisms to a level judged to beappropriate for a defined purpose and/or claim.4.Significance and Use4.1 This test method shall be used to determine if a chemi