1、Designation:E124712Standard Practice forDetecting Fluorescence in Object-Color Specimens bySpectrophotometry1This standard is issued under the fixed designation E1247;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last r
2、evision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice provides spectrophotometric methods fordetecting the presence of fluorescence in object-color speci-mens.NOTE1Since
3、 the addition of fluorescing agents(colorants,whiteningagents,etc.)is often intentional by the manufacturer of a material,information on the presence or absence of fluorescent properties in aspecimen may often be obtained from the maker of the material.1.2 This practice requires the use of a spectro
4、photometerthat both irradiates the specimen over the wavelength rangefrom 340 to 700 nm and allows the spectral distribution ofillumination on the specimen to be altered as desired.1.3 Within the above limitations,this practice is general inscope rather than specific as to instrument or material.1.4
5、 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Document
6、s2.1 ASTM Standards:2D2244 Practice for Calculation of Color Tolerances andColor Differences from Instrumentally Measured ColorCoordinatesE284 Terminology of AppearanceE308 Practice for Computing the Colors of Objects by Usingthe CIE SystemE313 Practice for Calculating Yellowness and WhitenessIndice
7、s from Instrumentally Measured Color CoordinatesE991 Practice for Color Measurement of Fluorescent Speci-mens Using the One-Monochromator MethodE1164 Practice for Obtaining Spectrometric Data for Object-Color EvaluationE1331 Test Method for Reflectance Factor and Color bySpectrophotometry Using Hemi
8、spherical GeometryE1348 Test Method for Transmittance and Color by Spec-trophotometry Using Hemispherical GeometryE1349 Test Method for Reflectance Factor and Color bySpectrophotometry Using Bidirectional(45:0 or 0:45)GeometryE2152 Practice for Computing the Colors of FluorescentObjects from Bispect
9、ral Photometric DataE2153 Practice for Obtaining Bispectral Photometric Datafor Evaluation of Fluorescent Color3.Terminology3.1 The definitions in Terminology E284,Practices E991,E2152,and E2153 are applicable to this practice.4.Significance and Use4.1 Several standards,including Practices E991,E116
10、4,andTest Methods E1331,E1348 and E1349,require either thepresence or absence of fluorescence exhibited by the specimenfor correct application.This practice provides spectrophoto-metric procedures for identifying the presence of fluorescencein materials.4.2 This practice is applicable to all object-
11、color specimens,whether opaque,translucent,or transparent,meeting the re-quirements for specimens in the appropriate standards listed in2.1.Translucent specimens should be measured by reflectance,with a standard non-fluorescent backing material,usually butnot necessarily black,placed behind the spec
12、imen duringmeasurement.4.3 This practice requires the use of a spectrophotometer inwhich the spectral distribution of the illumination on thespecimen can be altered by the user in one of several ways.Themodification of the illumination can either be by the insertionof optical filters between the ill
13、uminating source and thespecimen,without interfering with the detection of the radia-tion from the specimen,or by interchange of the illuminatingand detecting systems of the instrument or by scanning of boththe illuminating energy and detection output as in the two-monochromator method.1This practic
14、e is under the jurisdiction of ASTM Committee E12 on Color andAppearance and is the direct responsibility of Subcommittee E12.05 on Fluores-cence.Current edition approved July 1,2012.Published September 2012.Originallyapproved in 1988.Last previous edition approved in 2003 as E1247 03 which waswithd
15、rawn January 2012 and reinstated in July 2012.DOI:10.1520/E1247-12.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website
16、.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 4.4 The confirmation of the presence of fluorescence ismade by the comparison of spectral curves,color difference,orsingle parameter difference such as Y between the measure-ments.NOTE2In editions of E1247-92 and earlier,the test of fluorescencewas the two sets of spectral transmittances or radiance factor(reflectancefactors)differ by 1%of full scale at the wavelength of greatest differ