1、Designation:E109291(Reapproved 2010)Standard Specification forGlass Micro Folin Pipet,Disposable1This standard is issued under the fixed designation E1092;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A nu
2、mber in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification covers a glass disposable micro Folinpipet suitable for use in micro techniques for estimation ofblood sugar by the Folin
3、method.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.3 The following precautionary statement pertains only tothe test method portion,Section 8,of this specification.Thisstandard does not purport to address all of the safe
4、ty concerns,if any,associated with its use.It is the responsibility of the userof this standard to establish appropriate safety and healthpractices and determine the applicability of regulatory limita-tions prior to use.2.Referenced Documents2.1 ASTM Standards:2E438 Specification for Glasses in Labo
5、ratory Apparatus2.2 ISO Standard:1769 Laboratory GlasswarePipettesColor Coding33.Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 accuracy,nthe expected distribution of mean vol-umes around the stated volume.3.1.2 coeffcient of variation,nthe expected distribution ofindividual vol
6、umes around the mean volume.3.1.3 disposable,nmicro Folin pipets that are intended tobe used once only and then discarded.NOTE1Such pipets will only be expected to provide their specifiedperformance during the original operation.4.Classification4.1 This specification covers only one glass pipet as i
7、llus-trated in Fig.X1.1.5.Materials and Manufacture5.1 The pipet shall be made of borosilicate glass,Type 1,Class B,or soda lime glass,Type 2,in accordance withSpecification E438.6.Physical Properties6.1 DesignThe micro Folin pipet shall be made of onepiece construction glass tubing that is straight
8、 and uniform borewith a point pulled on one end to the dimensions as specifiedin Fig.X1.1.6.2 DimensionsThe pipet shall be made of tubing with anoutside diameter(o.d.)of 2.5 6 0.1 mm with an insidediameter(i.d.)of 1.8 6 0.1 mm.The uniformity of the boreshall be 60.05 mm throughout the straight porti
9、on of the pipet.The pipet shall be 183 6 1 mm long with all dimensions andtolerances as shown in Fig.X1.1.6.3 CapacityThe pipet shall be calibrated“to contain”(T.C.)0.1 and 0.2 mL at 20C.Marking shall be as specified in6.5.6.3.1 Accuracy(see 3.1.1)The accuracy from stated vol-ume shall be 61.0%for t
10、he 0.1 and 0.2-mL capacity and shallbe determined as specified in 8.1.6.3.2 Coeffcient of Variation(see 3.1.2)The coefficient ofvariation from stated volume for the 0.1 and 0.2-mL capacityshall not exceed 1.25%and shall be determined as specified in8.1.6.4 Graduation LinesThe pipet shall be calibrat
11、ed andmarked with graduation lines at 0.1 and 0.2 mL from the tip ofthe pipet.The graduation lines shall be 0.3 6 0.1 mm and shallcompletely encircle the tube.6.5 Pipet NomenclatureThe pipet shall be marked with0.1 and 0.2-mL markings slightly above the graduation lines.The pipet may be marked with
12、the inscription TC 20C,or themanufacturers or vendors name or trademark,or both.6.6 Color CodingThe pipet shall be color coded forcapacity as specified in ISO Standard 1769 with an“orange”1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct res
13、ponsibility of Subcommittee E41.01 onApparatus.Current edition approved Sept.1,2010.Published September 2010.Originallyapproved in 1986.Last previous edition approved in 2005 as E1092 91(2005).DOI:10.1520/E1092-91R10.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM C
14、ustomer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from International Organization for Standardization(ISO),1,ch.dela Voie-Creuse,Case postale 56,CH-1211,Geneva 20,Switzerland,http:/www.iso
15、.ch.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 color band that is 6 6 2 mm wide and located 16 6 2 mm fromthe top of the pipet as shown in Fig.X1.1.6.7 Marking PermanencyGraduation lines,inscriptions,and numerical markings on the pip
16、et(other than the color codeband)shall be black in color.All markings may not be ofpermanent nature but must possess sufficient stability to endurenormal transportation and its expected one-time use and mustmeet the test requirements as specified in 8.5.6.8 Lot ControlA lot or control number shall be indicatedon the pipet container package.This lot or control number shallbe traceable to the origin and purchase order of raw materialglass tubing.7.Workmanship,Finish,and Appearance7.1 The pipet sha