1、Designation:E119919Standard Practice forSampling Zooplankton with a Clarke-Bumpus PlanktonSampler1This standard is issued under the fixed designation E1199;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A n
2、umber in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice covers the procedures for obtaining quan-titative samples of a zooplankton community by use of aClarke-Bumpus plankton sampler
3、.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-p
4、riate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelop
5、ment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D4134 Practice for Sampling Phytoplankton with a Clarke-Bumpus Plankton SamplerE1200 Practice for Preserving Zooplankt
6、on Samples3.Summary of Practice3.1 The sampler is towed from a moving boat at a specifieddepth.The sampler uses a net for the collection and concen-tration of zooplankton.The actual volume of water entering thesampler is measured by a calibrated flowmeter.The zooplank-ton are preserved as dictated b
7、y the objectives of the study.4.Significance and Use4.1 The advantages of the Clarke-Bumpus plankton samplerare as follows:4.1.1 It will sample a discrete depth or multiple depths,depending upon the sampling design.4.1.2 It is a slow to medium speed sampler requiring atowing speed of three to five k
8、nots.4.1.3 The sample size can be easily controlled.4.1.4 The sampler is lightweight and can be used withoutauxiliary equipment.4.1.5 It has a relatively high filtration efficiency factor of0.88.4.1.6 It is a versatile sampler and can be used in all but theshallowest waters.4.1.7 The flowmeter recor
9、ds the amount of water that passesinto the net.4.1.8 Overspill of water at the mouth of the net due toexcess speed of towing is of minimal consequence.4.2 The disadvantages of the Clarke-Bumpus plankton sam-pler are as follows:4.2.1 The flowmeter requires frequent maintenance includ-ing calibration
10、and lubrication.4.2.2 It is not suitable for use in very small areas or shallowwaters.4.3 There are several special considerations that shall beobserved when using a Clarke-Bumpus plankton sampler.Theyare:4.3.1 The flowmeter should be calibrated and servicedfrequently to ensure efficient and accurat
11、e operation.4.3.2 The sampler is relatively fragile,particularly theclosing device and flowmeter.This necessitates careful deploy-ment and recovery procedures.4.3.3 Following each collection,the net must be thoroughlywashed.4.3.4 Special attention must be given to the strength of thecable and its at
12、tachment to avoid loss of the sampler.4.3.5 The sampler should not be used in beds ofmacrophytes,in waters containing submerged objects,or closeto the bottom.4.3.6 The net should be inspected frequently for pin-sizeholes,tears,net deterioration,and other anomalies.1This practice is under the jurisdi
13、ction of ASTM Committee D19 on Water andis the direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved April 1,2019.Published April 2019.Originallyapproved in 1987.Last previous edition approved in 2012 as E1199 87(2012).DOI:10.1520/E1199-19.2For referenced ASTM s
14、tandards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 1942
15、8-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technica
16、l Barriers to Trade(TBT)Committee.1 4.3.7 Following use,the wet net should be suspended fulllength in the air in subdued light and allowed to dry.5.Apparatus5.1 The Clarke-Bumpus plankton sampler is a 12.7-cmdiameter by 15.2-cm long brass tube consisting of a metalframe in which an interchangeable conical plankton net isattached at the mouth and at the cod end.3This sampler isavailable in three sizes:12,20,and 30 cm.The most widelyused size has been the 20-cm diameter net which reportedly hasbet