1、Designation:E151095(Reappproved 2013)1Standard Practice forInstalling Fused Silica Open Tubular Capillary Columns inGas Chromatographs1This standard is issued under the fixed designation E1510;the number immediately following the designation indicates the year oforiginal adoption or,in the case of r
2、evision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1NOTEWarning statements were editorially corrected in January 2013.1.Scope1.1 This practice covers the installatio
3、n and maintenance offused silica capillary columns in gas chromatographs that arealready retrofitted for their use.This practice excludes infor-mation on:1.1.1 Injection techniques.1.1.2 Column selection.1.1.3 Data acquisition.1.1.4 System troubleshooting and maintenance.1.2 For additional informati
4、on on gas chromatography,please refer to Practice E260.For specific precautions,see7.2.2.2(1),7.2.2.2(2),7.2.7,and 7.2.7.2.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of t
5、hesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.For specific safetyinformation,see Section 6,7.2.2.2(1),7.2.2.2(2),7.2.7
6、,and7.2.7.2.22.Referenced Documents2.1 ASTM Standards:3E260 Practice for Packed Column Gas ChromatographyE355 Practice for Gas Chromatography Terms and Relation-ships2.2 CGA Publications:4CGA P-1 Safe Handling of Compressed Gases in ContainersCGA G-5.4 Standard for Hydrogen Piping Systems at Con-sum
7、er LocationsCGA P-9 The Inert Gases:Argon,Nitrogen and HeliumCGA V-7 Standard Method of Determining Cylinder ValveOutlet Connections for Industrial Gas MixturesCGA P-12 Safe Handling of Cryogenic LiquidsHB-3 Handbook of Compressed Gases3.Terminology3.1 Terms and relations are defined in Practice E35
8、5.3.2 Nomenclature for open tubular or capillary columnswith a bore of 0.75 mm or less:3.3 porous layer open tubular(PLOT)refers to columnswith particles attached on the inside wall consisting of copo-lymers such as styrene/divinylbenzene,molecular sieves,oradsorbents such as Al2O2in film thicknesse
9、s of 5 to 50 m.3.4 support coated open tubular(SCOT)refers to fineparticles(silica or fine diatomite)coated with liquid stationaryphase,which is then deposited on the inside column wall toimprove stationary phase stability and sample capacity.3.5 wall coated open tubular(WCOT)refers to columnscoated
10、 on the inside wall with a liquid stationary phase in filmthicknesses of 0.1 to 10.0 m.Also referred to as FSOT orfused silica open tubular.4.Summary of Practice4.1 The packed gas chromatography system is described inPractice E260 and is essentially the same as a capillary gaschromatography system e
11、xcept for modifications to the injectorand detector to accommodate the low flow rates and samplecapacity associated with capillary columns.Refer to the gaschromatography(GC)instrument manual for specific details oninjector or detector pneumatics for capillary columns.4.2 Prior to performing a capill
12、ary GC analysis,the capil-lary column configuration must be determined.The stationary1This practice is under the jurisdiction of ASTM Committee E13 on MolecularSpectroscopy and Separation Science and is the direct responsibility of Subcom-mittee E13.19 on Separation Science.Current edition approved
13、Jan.1,2013.Published January 2013.Originallyapproved in 1993.Last previous edition approved in 2005 as E1510 95(2005).DOI:10.1520/E1510-95R13.2Reprinted by permission of Restek Corp.,110 Benner Circle,Bellefonte,PA16823-8812.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontac
14、t ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.4Available from Compressed Gas Association(CGA),4221 Walney Rd.,5thFloor,Chantilly,VA 20151-2923,http:/.Copyright ASTM International,100 Barr
15、 Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 phase type,stationary phase film thickness,column insidediameter,and column length must be selected.It is beyond thescope of this practice to provide these details.Consult acolumn or instrument supplier for details on selecting
16、 theappropriate capillary column configuration.4.3 Apply caution during handling or installation to avoidscratching or abrading the protective outer coating of thecolumn.Scratches or abrasions cause the fused silica capillarycolumn to spontaneously break or fail during usage.5.Significance and Use5.1 This practice is intended to be used by all analysts usingfused silica capillary chromatography.It contains the recom-mended steps for installation,preparation,proper installation,and continued colu