1、Designation:E146392(Reapproved 2012)Standard Guide forConducting Static and Flow-Through Acute Toxicity TestsWith Mysids From the West Coast of the United States1This standard is issued under the fixed designation E1463;the number immediately following the designation indicates the year oforiginal a
2、doption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This guide describes procedures for obtaining dataconcerning the adverse eff
3、ects of a test material(not food)added to marine and estuarine waters on certain species ofmarine and estuarine mysids during 96 h of continuousexposure.Juvenile mysids used in these tests are taken fromcultures shortly after release from the brood and exposed tovarying concentrations of a toxicant
4、in static or flow-throughconditions.These procedures will be useful for conductingtoxicity tests with other species of mysids,although modifica-tions might be necessary.1.2 Modifications of these procedures might be justified byspecial needs or circumstances.Although using appropriateprocedures is m
5、ore important than following prescribedprocedures,results of tests conducted using unusual proceduresare not likely to be comparable to results of many other tests.Comparisons of results obtained using modified and unmodi-fied versions of these procedures might provide useful infor-mation concerning
6、 new concepts and procedures for conduct-ing acute tests with other species of mysids.1.3 The procedures given in this guide are applicable tomost chemicals,either individually or in formulations,com-mercial products,and known or unknown mixtures.Withappropriate modifications these procedures can be
7、 used toconduct acute tests on factors such as temperature,salinity,anddissolved oxygen.These procedures can also be used to assessthe toxicity of potentially toxic discharges such as municipalwastes,oil drilling fluids,produced water from oil wellproduction,and other types of industrial wastes.1.4
8、Results of acute toxicity tests with toxicants experimen-tally added to salt and estuarine waters should usually bereported in terms of a LC50(median lethal concentration).1.5 This guide is arranged as follows:SectionReferenced Documents2Terminology3Summary of Guide4Significance and Use5Apparatus6Fa
9、cilities6.1Construction Materials6.2Metering Systems6.3Test Chambers6.4Cleaning6.5Acceptability6.6Safety Precautions7Dilution Water8Requirements8.1Source8.2Treatment8.3Characterization8.4Test Material9General9.1Stock Solution9.2Test Concentrations9.3Test Organisms10Species10.1Age10.2Source10.3Brood
10、Stock10.4Food10.5Handling10.6Harvesting Young10.7Quality10.8Procedure11Experimental Design11.1Dissolved Oxygen11.2Temperature11.3Loading11.4Salinity11.5Light11.6Beginning of Test11.7Feeding11.8Duration of Test11.9Biological Data11.10Other Measurements11.11Analytical Methodology12Acceptability of Tes
11、t13Interpretation of Results14Report15AppendixesHolmesimysis costataX1Neomysis mercedisX21This guide is under the jurisdiction ofASTM Committee E50 on EnvironmentalAssessment,Risk Management and Corrective Action and is the direct responsibil-ity of Subcommittee E50.47 on Biological Effects and Envi
12、ronmental Fate.Current edition approved Dec.1,2012.Published January 2013.Originallyapproved in 1992.Last previous edition approved in 2004 as E1463 92(2004).DOI:10.1520/E1463-92R12.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 1.6 The
13、values stated in SI units are to be regarded as thestandard.1.7 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bili
14、ty of regulatory limitations prior to use.Specific precau-tionary statements are given in Section 7.2.Referenced Documents2.1 ASTM Standards:2E729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating to Biological Effects
15、and Envi-ronmental FateE1023 Guide for Assessing the Hazard of a Material toAquatic Organisms and Their UsesE1191 Guide for Conducting Life-Cycle Toxicity Tests withSaltwater MysidsE1192 Guide for Conducting Acute Toxicity Tests on Aque-ous Ambient Samples and Effluents with Fishes,Macroinvertebrate
16、s,and AmphibiansE1203 Practice for Using Brine Shrimp Nauplii as Food forTest Animals in Aquatic Toxicology(Withdrawn 2013)3IEEE/ASTM SI 10 American National Standard for Use ofthe International System of Units(SI):The Modern MetricSystem3.Terminology3.1 The terms“must,”“should,”“may,”“can,”and“might”have very specific meanings in this guide.“Must”is used toexpress the strongest possible recommendation,just short of anabsolute requirement,that is,to state that this test ought to bedesigned to sa