1、Designation:E215301(Reapproved 2011)Standard Practice forObtaining Bispectral Photometric Data for Evaluation ofFluorescent Color1This standard is issued under the fixed designation E2153;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revisi
2、on,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.INTRODUCTIONThe fundamental procedure for evaluating the color of a fluorescent specimen is to obtain bispectralphotome
3、tric data for specified irradiating and viewing geometries,and from these data to computetristimulus values based on a CIE(International Commission on Illumination)standard observer anda CIE standard illuminant.The considerations involved and the procedures used to obtain precisebispectral photometr
4、ic data are contained in this practice.Values and procedures for computing CIEtristimulus values from bispectral photometric data are contained in Practice E2152.Generalconsiderations regarding the selection of appropriate irradiating and viewing geometries are containedin Guide E179;further specifi
5、c considerations applicable to fluorescent specimens are contained in thispractice.1.Scope1.1 This practice addresses the instrumental measurementrequirements,calibration procedures,and material standardsneeded for obtaining precise bispectral photometric data forcomputing the colors of fluorescent
6、specimens.1.2 This practice lists the parameters that must be specifiedwhen bispectral photometric measurements are required inspecific methods,practices,or specifications.1.3 This practice applies specifically to bispectrometers,which produce photometrically quantitative bispectral data asoutput,us
7、eful for the characterization of appearance,as op-posed to spectrofluorimeters,which produce instrument-dependent bispectral photometric data as output,useful for thepurpose of chemical analysis.1.4 The scope of this practice is limited to the discussion ofobject-color measurement under reflection g
8、eometries;it doesnot include provisions for the analogous characterization ofspecimens under transmission geometries.1.5 This standard may involve hazardous materials,operations,and equipment.This standard does not purport toaddress all of the safety concerns,if any,associated with itsuse.It is the
9、responsibility of the user of this standard toestablish appropriate safety and health practices and deter-mine the applicability of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2E179 Guide for Selection of Geometric Conditions forMeasurement of Reflection and Transmis
10、sion Propertiesof MaterialsE284 Terminology of AppearanceE925 Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Bandwidthdoes not Exceed 2 nmE958 Practice for Measuring Practical Spectral Bandwidthof Ultraviolet-Visible SpectrophotometersE1164 Practice
11、for Obtaining Spectrometric Data for Object-Color EvaluationE1341 Practice for Obtaining Spectroradiometric Data fromRadiant Sources for ColorimetryE2152 Practice for Computing the Colors of FluorescentObjects from Bispectral Photometric Data2.2 NPL Publications:NPL Report MOM 12 Problems of spectro
12、fluorimetric stan-dards for reflection and colorimetric use31This practice is under the jurisdiction of ASTM Committee E12 on Color andAppearance and is the direct responsibility of Subcommittee E12.05 on Fluores-cence.Current edition approved Nov.1,2011.Published November 2011.Originallyapproved in
13、 2001.Last previous edition approved in 2006 as E2153-01(2006).DOI:10.1520/E2153-01R11.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary pag
14、e onthe ASTM website.3Available from National Physical Laboratory,Queens Road,Teddington,Middlesex,United Kingdom TW11 0LW,http:/www.npl.co.uk/.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 2.3 CIE Publications:CIE No.38 Radiometric and
15、 Photometric Characteristics ofMaterials and Their Measurement4CIE No.15.2 Colorimetry,2nd Edition4CIE Report of TC-2.25:Calibration Methods and Photolu-minescent Standards for Total Radiance Factor Measure-ment42.4 NIST Publications:NBS No.260-66 Didymium Glass Filters for Calibrating theWavelength
16、 Scale of Spectrophotometers53.Terminology3.1 DefinitionsThe definitions contained in TerminologyE284 are applicable to this practice.3.2 Definitions of Terms Specific to This Standard:3.2.1 bispectral fluorescence radiance factor,bF(),ntheratio of the spectral radiance at wavelength due to fluores-cence from a point on the specimen when irradiated atwavelength to the total radiance of the perfectly reflectingdiffuser similarly irradiated and viewed(see NPL ReportMOM 12).3.2.2 bispectral radianc