1、Designation:D478301(Reapproved 2013)Standard Test Methods forResistance of Adhesive Preparations in Container to Attackby Bacteria,Yeast,and Fungi1This standard is issued under the fixed designation D4783;the number immediately following the designation indicates the year oforiginal adoption or,in t
2、he case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.This standard has been approved for use by agencies of the Department of Defense.1.Scope1.1 These test
3、 methods cover the determination of theresistance of liquid adhesive preparations to microbial attack inthe container by challenging adhesive specimens with culturesof bacteria,yeast,or fungi,and checking for their ability toreturn to sterility.These test methods return qualitative results.1.2 The v
4、alues stated in SI units are to be regarded as thestandard.The values in parentheses are for information only.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety a
5、nd health practices and determine the applica-bility of regulatory limitations prior to use.These test methodsare designed to be used by persons trained in correct micro-biological technique.Specific precautionary statements aregiven in Section 8.2.Referenced Documents2.1 ASTM Standards:2D907 Termin
6、ology of AdhesivesD4299 Test Method for Effect of Bacterial Contamination onPerformance of Adhesive Preparations and AdhesivesFilms(Withdrawn 1990)3D4300 Test Methods for Ability of Adhesive Films toSupport or Resist the Growth of FungiE640 Test Method for Preservatives in Water-ContainingCosmeticsN
7、OTE1Test Method E640 is under the jurisdiction of ASTMCommittee E35 on Pesticides.The procedure in this method outlines aserial dilution method of determining plate count using a pour platetechnique.2.2TAPPI Method:T 487 Fungus Resistance of Paper and Paperboard42.3 CSMA:Cosmetics Preservation,Metho
8、d 3853.Terminology3.1 DefinitionsMany terms in these test methods aredefined in Terminology D907.3.2 Definitions of Terms Specific to This Standard:3.2.1 adhesive preparation,nthe adhesive as packaged fordistribution,storage,and use.3.3 Abbreviations:3.3.1 PBSphosphate buffered saline.3.3.2 PDApotat
9、o dextrose agar.3.3.3 YMPGyeast malt peptone glucose(agar).4.Summary of Test Method4.1 The adhesive specimen is challenged by inoculationwith a culture of bacteria,yeast,or fungi,which may be asingle species or a mixed culture of several species,followingthe guidelines given in Note 6.The inoculated
10、 adhesivespecimen is stored at 21 to 27C(70 to 80F)for 7 days,duringwhich time cultures(streak plates)are made at preset intervals.See Note 2.At any point in the series of challenges,if theinoculated specimen shows microbial growth on the streakplates made during the week following the challenge(ind
11、icat-ing that it has not returned to sterility),the test is discontinued,and the sample is reported as not resistant to attack in thecontainer by the species or combination of species used as theinoculum.If the cultures show no growth,the test is repeatedwith up to four challenges.If the specimen te
12、sts out as sterilefollowing the fourth challenge,it is reported to be resistant toattack in the container by the species or combination of speciesof bacteria,fungi,or yeast used as the inoculum.At the1These test methods are under the jurisdiction of ASTM Committee D14 onAdhesives and are the direct
13、responsibility of Subcommittee D14.30 on WoodAdhesives.Current edition approved Oct.1,2013.Published October 2013.Originallyapproved in 1988.Last previous edition approved in 2008 as D4783 01(2008).DOI:10.1520/D4783-01R13.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact A
14、STM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.4Available from Technical Association of the Pulp and Paper
15、Industry(TAPPI),15 Technology Parkway South,Norcross,GA 30092,http:/www.tappi.org.5This method is the same as Test Method E640.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 discretion of the biological laboratory,the test may be discon-
16、tinued after the second or third challenge.See Section 16 forfurther interpretation.4.2 The time necessary to kill is determined by noting theearliest streak plate to read sterile.If the 4-h plate is positiveand the 24-h plate is negative,the kill time could be narroweddown further by repeating the challenge and making streakplates at intervals of 4,8,12,and 24 h following the challenge.4.3 The testing laboratory has the option of changing thetiming of the challenges,the sterility checks,and the