1、Designation:D478301(Reapproved 2021)Standard Test Methods forResistance of Adhesive Preparations in Container to Attackby Bacteria,Yeast,and Fungi1This standard is issued under the fixed designation D4783;the number immediately following the designation indicates the year oforiginal adoption or,in t
2、he case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.This standard has been approved for use by agencies of the U.S.Department of Defense.1.Scope1.1 These
3、test methods cover the determination of theresistance of liquid adhesive preparations to microbial attack inthe container by challenging adhesive specimens with culturesof bacteria,yeast,or fungi,and checking for their ability toreturn to sterility.These test methods return qualitative results.1.2 T
4、he values stated in SI units are to be regarded as thestandard.The values in parentheses are for information only.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safe
5、ty,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.These test methods are designed to be used by persons trainedin correct microbiological technique.Specific precautionarystatements are given in Section 8.1.4 This international standard was
6、developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Doc
7、uments2.1 ASTM Standards:2D907 Terminology of AdhesivesD4299 Test Method for Effect of Bacterial Contamination onPerformance of Adhesive Preparations and AdhesivesFilms(Withdrawn 1990)3D4300 Test Methods for Ability of Adhesive Films toSupport or Resist the Growth of FungiE640 Test Method for Preser
8、vatives in Water-ContainingCosmeticsNOTE1Test Method E640 is under the jurisdiction of ASTMCommittee E35 on Pesticides.The procedure in this method outlines aserial dilution method of determining plate count using a pour platetechnique.2.2TAPPI Method:4T 487 Fungus Resistance of Paper and Paperboard
9、2.3 CSMA:Cosmetics Preservation,Method 3853.Terminology3.1 DefinitionsMany terms in these test methods aredefined in Terminology D907.3.2 Definitions of Terms Specific to This Standard:3.2.1 adhesive preparation,nthe adhesive as packaged fordistribution,storage,and use.3.3 Abbreviations:3.3.1 PBSpho
10、sphate buffered saline.3.3.2 PDApotato dextrose agar.3.3.3 YMPGyeast malt peptone glucose(agar).4.Summary of Test Method4.1 The adhesive specimen is challenged by inoculationwith a culture of bacteria,yeast,or fungi,which may be asingle species or a mixed culture of several species,followingthe guid
11、elines given in Note 6.The inoculated adhesivespecimen is stored at 21 to 27C(70 to 80F)for 7 days,duringwhich time cultures(streak plates)are made at preset intervals.See Note 2.At any point in the series of challenges,if theinoculated specimen shows microbial growth on the streakplates made during
12、 the week following the challenge(indicat-ing that it has not returned to sterility),the test is discontinued,1These test methods are under the jurisdiction of ASTM Committee D14 onAdhesives and are the direct responsibility of Subcommittee D14.30 on WoodAdhesives.Current edition approved April 1,20
13、21.Published April 2021.Originallyapproved in 1988.Last previous edition approved in 2013 as D4783 01(2013).DOI:10.1520/D4783-01R21.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume informatio
14、n,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.4Available from Technological Association of the Pulp and Paper Industry(TAPPI),15 Technology Parkway South,Suite 115,Peachtree Corners,GA 30092,http:
15、/www.tappi.org.5This method is the same as Test Method E640.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in
16、 the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1and the sample is reported as not resistant to attack in thecontainer by the species or combination of species used as theinoculum.If the cultures show no growth,the test is repeatedwith up to four challenges.If the specimen tests out as sterilefollowing the fourth challenge,it is reported to be resistant toattack i