1、Designation:E279917Standard Test Method forTesting Disinfectant Efficacy against Pseudomonasaeruginosa Biofilm using the MBEC Assay1This standard is issued under the fixed designation E2799;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revi
2、sion,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method specifies the operational parametersrequired to grow and treat a Pseudomonas aeruginosa b
3、iofilmin a high throughput screening assay known as the MBEC(trademarked)2(Minimum Biofilm Eradication Concentration)Physiology and Genetics Assay.The assay device consists of aplastic lid with ninety-six(96)pegs and a correspondingreceiver plate with ninety-six(96)individual wells that have amaximu
4、m 200 L working volume.Biofilm is established onthe pegs under batch conditions(that is,no flow of nutrientsinto or out of an individual well)with gentle mixing.Theestablished biofilm is transferred to a new receiver plate fordisinfectant efficacy testing.3,4The reactor design allows forthe simultan
5、eous testing of multiple disinfectants or onedisinfectant with multiple concentrations,and replicatesamples,making the assay an efficient screening tool.1.2 This test method defines the specific operational param-eters necessary for growing a Pseudomonas aeruginosabiofilm,although the device is vers
6、atile and has been used forgrowing,evaluating and/or studying biofilms of differentspecies as seen in Refs(1-4).51.3 Validation of disinfectant neutralization is included aspart of the assay.1.4 This test method describes how to sample the biofilmand quantify viable cells.Biofilm population density
7、is re-corded as log10colony forming units per surface area.Efficacyis reported as the log10reduction of viable cells.1.5 Basic microbiology training is required to perform thisassay.1.6 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstan
8、dard.1.7 ASTM International takes no position respecting thevalidity of any patent rights asserted in connection with anyitem mentioned in this standard.Users of this standard areexpressly advised that determination of the validity of any suchpatent rights,and the risk of infringement of such rights
9、,areentirely their own responsibility.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limit
10、ations prior to use.1.9 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization T
11、echnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:6E1054 Test Methods for Evaluation of Inactivators of Anti-microbial Agents2.2 Other Standards:Method 9050 C.1.a Buffered Dilution Water Preparationaccording to Rice et al(5)3.Terminology3.1 Definitions:1This test metho
12、d is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved April 1,2017.Published May 2017.Originallyapproved in 2011.Last previous edition approv
13、ed in 2012 as E2799 12.DOI:10.1520/E279917.2The MBEC trademark is held by Innovotech,Inc.,Edmonton,Alberta,Canada.3The sole source of supply of the apparatus known to the committee at this timeis Innovotech Inc.,Edmonton,Alberta,Canada.If you are aware of alternativesuppliers,please provide this inf
14、ormation to ASTM International Headquarters.Your comments will receive careful consideration at a meeting of the responsibletechnical committee,1which you may attend.4The MBECAssay is covered by a patent.Interested parties are invited to submitinformation regarding the identification of an alternati
15、ve(s)to this patented item tothe ASTM International Headquarters.Your comments will receive careful consid-eration at a meeting of the responsible technical committee,1which you may attend.5The boldface numbers in parentheses refer to a list of references at the end ofthis standard.6For referenced A
16、STM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopm