1、Designation:D266795(Reapproved 2008)Standard Test Method forBiodegradability of Alkylbenzene Sulfonates1This standard is issued under the fixed designation D2667;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revisi
2、on.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.This standard has been approved for use by agencies of the Department of Defense.1.Scope1.1 This test method2covers the determination of the degr
3、eeof biodegradability of alkylbenzene sulfonates.It serves as anindex of the suitability of the sulfonate for general use as asurfactant.1.2 In general,this test method distinguishes between sul-fonates in which the alkyl side chains are linear and those inwhich they are branched,since the former ar
4、e more readilybiodegradable.If the alkylbenzene sulfonate in fully formu-lated products is to be examined,it must be extracted using themethod noted in Annex A1.(See Appendix X1 for data.)1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is the
5、responsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.Material SafetyData Sheets are available for reagents and materials.Reviewthem for hazards prior to usage.2.Referenced Documents2.
6、1 ASTM Standards:3E1298 Guide for Determination of Purity,Impurities,andContaminants in Biological Drug ProductsD2330 Test Method for Methylene Blue Active Substances(Withdrawn 2011)4E1625 Test Method for Determining Biodegradability ofOrganic Chemicals in Semi-Continuous Activated Sludge(SCAS)3.Sum
7、mary of Test Method3.1 The sample is first subjected to a presumptive test basedon shake culture.When necessary,the sample may be sub-jected to a confirming test based on semicontinuous treatmentwith activated sludge.3.2 In the presumptive test,microorganisms are inoculatedinto a flask that contains
8、 a chemically defined microbial growthmedium(basal medium)and the surfactant to be tested.Aeration is accomplished by continuous shaking of the flask.Following two adaptive transfers,biodegradation is determinedby measuring the reduction in surfactant content during the testperiod.3.3 In the confirm
9、ing test,activated sludge obtained from asewage treatment plant is used.The sludge,the surfactant to betested,and a synthetic sewage used as an energy source for thesludge microorganisms are all placed in a specially designedaeration chamber.The mixture is aerated for 23 h,allowed tosettle,and the s
10、upernatant material removed.The sludgeremaining in the aeration chamber is then brought back tovolume with fresh surfactant and synthetic sewage and thecycle repeated.Biodegradation is determined by the reductionin surfactant content during each cycle.4.Significance and Use4.1 This test method is de
11、signed to determine whether thesulfonate tested will be removed sufficiently by usual methodsof sewage treatment for the effluent to be safely discharged tothe environment without further treatment.4.2 If the surfactant reduction in the presumptive test equalsor exceeds 90%,the material is considere
12、d to be adequatelybiodegradable without further testing.4.3 If the surfactant reduction in the presumptive test isbetween 80 and 90%,the material should be subjected to theconfirming test.4.4 If the surfactant reduction in the presumptive test isbelow 80%,the material is considered inadequately biod
13、e-gradable.1This test method is under the jurisdiction of ASTM Committee E47 onBiological Effects and Environmental Fateand is the direct responsibility ofSubcommittee E47.04 on Environmental Fate and Transport of Biologicals andChemicals.Current edition approved Feb.1,2008.Published April 2008.Orig
14、inallyapproved in 1967.Last previous edition approved in 2001 as D2667 95(2001).DOI:10.1520/D2667-95R08.2This test method is based on“AProcedure and Standards for the Determinationof the Biodegradability of Alkyl Benzene Sulfonate and Linear Alkylate Sulfonate”by the Committee on Biodegradation Test
15、 Methods of the Soap and the DetergentAssociation,Journal of the Americal Oil Chemists Society,Vol 42,1965,p.986.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the stan
16、dards Document Summary page onthe ASTM website.4The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesNOTICE:This standard has either been superseded and replaced by a new version or withdrawn.Contact ASTM International(www.astm.org)for the latest information14.5 If it is necessary to run the confirming test,the surfactantreduction in this test must be at least