1、SnapShot:Tight and Adherens Junction SignalingJennifer L.Harder and Ben MargolisDepartments of Internal Medicine and Biological Chemistry,University of Michigan,Ann Arbor,MI 48109,USASee online version for legend,abbreviations,and references.1118 Cell 133,June 13,2008 2008 Elsevier Inc.DOI 10.1016/j
2、.cell.2008.06.0021118.e1 Cell 133,June 13,2008 2008 Elsevier Inc.DOI 10.1016/j.cell.2008.06.002SnapShot:Tight and Adherens Junction SignalingJennifer L.Harder and Ben MargolisDepartments of Internal Medicine and Biological Chemistry,University of Michigan,Ann Arbor,MI 48109,USASignaling pathways con
3、verging upon and emanating from adherens junctions(AJs)and tight junctions(TJs)affect many cellular functions,including cytoskeletal dynamics,polarized vesicle trafficking,protein synthesis,and proliferation.Many pathologic processes target junctional proteins,resulting in the disassembly of junc-ti
4、ons and breakdown of tissue integrity.As signaling pathways and protein components of junctions vary among organisms,this SnapShot highlights those pathways established in mammalian epithelia.Assembly of Apical Junctions and Polarized MembranesTransdimerization of heterophilic nectins between adjace
5、nt cells is a critical event leading to the development of AJs,TJs,and polarized membrane domains.(1)Upon as-sociation with dimerized nectin,integrin V3 initiates a set of signaling events involving c-Src,which results in the activation of Rap1,Cdc42,and Rac.Subsequently,Rap1 complexes with afadin a
6、nd p120ctn resulting in stabilization of E-cadherin at the cell surface followed by recruitment of-and-catenin and Fer kinase.E-cadherin stabiliza-tion also triggers delivery of occludin and claudin to nascent TJs at the apical side of AJs.Concurrently,nectin accumulates JAM through its interaction
7、with afadin and ZO1,developing a platform for Par3 localization.Par6 and aPKC join Par3 after LIMK is released,an event triggered by phosphorylation of Par3 downstream of EGFR activation.(2)aPKC at nascent TJs contributes to polarization of the apical membrane by targeting specific proteins for excl
8、usion from this region.Phosphorylation of Par1 and mLgl by aPKC results in their recruitment to the nascent BLM.At the BLM,Par1 and mLgl inhibit incursion of the apical Par complex through sequestration and phosphorylation of complex components.(3)PTEN is recruited to junctional and apical surfaces
9、where it generates PIP2,which attracts Anx-2.The latter interacts with the Par complex which in turn associates with the Crumbs polarity complex consisting of Crumbs3,PALS1,and PATJ,thus firmly defining the apical-junctional membrane.Junction Stabilization and MaintenanceMaturation and maintenance o
10、f both AJs and TJs requires the formation of an elaborate web of highly dynamic and specifically localized interactions with the cytoskeleton.Competing Control of Actin Dynamics(1)At AJs,tyrosine phosphorylation at one site on p120ctn by c-Src activates RhoA and promotes activity of myosin II throug
11、h mDia1 resulting in stabilization of AJs.These events are inhibited by Fyn-directed tyrosine phosphorylation of another site on p120ctn.(2)IQGAP and-catenin are rival binding partners for-catenin at AJs.By prevent-ing-catenins recruitment,IQGAP effectively blocks AJ anchorage to F-actin.Activated R
12、ac/Cdc42 inhibit IQGAPs interaction with-catenin,thus favoring-catenins association with proteins at the AJ.The presence of-catenin triggers recruitment of other proteins that bind F-actin,culminating in maturation of AJ-actin cytoskeleton interaction.(3)At TJs,the Par complex is dependent on Rho GT
13、Pases to assemble and activate resident components.However,Par3 also has a negative feedback func-tion;it inhibits activity of the Rac GEF Tiam1 thus preventing the propagation of locally activated Rac.Likewise,the RhoA GEF GEF-HI is activated by aPKC but inhibited by cingulin,which resides at the T
14、J in association with ZO1.Regionalized Signaling at JunctionsLocalized activation of actin effectors spatially regulates actin dynamics differentially at AJs and TJs.(4)At AJs,Rac activated by resident PI3K recruits cortactin,which is then activated by Fer kinase bound to p120ctn.At TJs,ZO1 recruits
15、 a Cdc42 GEF,Tuba,resulting in activation of N-WASP.Localized activation of VASP,Mena,and gelsolin also occurs upon junction formation.Upstream signals also negatively regulate modulators of actin;at TJs,activated Rab13 inhibits PKAs activation of VASP,blocking its interaction with actin and prevent
16、ing the assembly of TJ components.Interactions with Microtubules(5)Downstream of LKB1,activated Par1 phosphorylates MAPs,which results in their release from microtubules allowing for motors to move their cargos in an unhindered fashion.Additionally,interactions between p120ctn and kinesin as well as
17、-catenin and dynein are described.Directed Trafficking of Vesicles Carrying Membrane Components(6)When Rich1,a Cdc42 GAP,is active at TJs,it assists in the redirection of endocytosed occludin and claudin back to the cell surface through the apical recycling pathway.When Rich1 is inactivated by AMOT,
18、endocytosed proteins are directed for degradation instead.In turn,AMOTs activity is negatively regulated by the formation of AJs.Regulation of Proliferation and DifferentiationThe establishment of AJs and TJs in normal epithelia represses pathways for proliferation and promotes those for differentia
19、tion.(1)At high cell density,ZO1 sequesters ZONAB and CDK4 from the nucleus and shuttles them to TJs,preventing transcriptional control by ZONAB and entry into S phase triggered by CDK4.At low cell density,ZONAB localizes to the nucleus,complexes with symplekin,and regulates transcription.RalA also
20、binds and regulates ZONAB in a manner similar to ZO1.(2)PTEN is recruited to both TJs and AJs through its interactions with MAGI proteins.At AJs,this interaction is stabilized by the interaction of MAGI with vinculin.At both junctions,PTENs ability to suppress PI3K/Akt signaling is enhanced by bindi
21、ng to MAGI proteins,thereby repressing proliferation,preventing transcriptional repression of E-cadherin,and blocking events leading to loss of AB polarity.(3)When-catenin is phosphorylated on specific tyrosine residues,it dissociates from E-cadherin and becomes part of a soluble cytoplasmic pool wh
22、ere it is targeted for degradation by the APC/Axin/GSK3 complex.Wnt signaling inhibits-catenin degradation,which allows-catenin to translocate to the nucleus where it binds TCF transcription factors and promotes the transcription of wnt target genes.Pathologic Effects on Junction ComponentsProcesses
23、 such as wound healing and tissue maintenance dictate that cells maintain dynamic control of junction assembly and disassembly.Many pathologic pro-cesses such as cancer and invasion of host cells by microbes affect these pathways.(1)Adenoviral E4-ORF1 directly binds and prevents TJ localization of P
24、ATJ and ZO2,stimulates the PI3K/Akt signaling pathway,and indirectly alters localization of Par3,PALS1,and ZO1,thus disrupting TJs and AB polarity.Similarly,the HPV-18 E6 oncogene sequesters PATJ and its paralog MPDZ/MUPP1 as well as hScrib/mDlg and targets them for degradation,contributing to loss
25、of polarity.(2)The Heliobacter pylori protein cagA disrupts TJs and AB polarity.Upon entry into gastric epithelial cells,cagA is phosphorylated and activated by membrane-associated tyrosine kinases.It then interacts with Par1,preventing its phosphorylation by aPKC.Thus,Par1 remains pathologically as
26、sociated with apical membranes where it interferes with proper membrane polarization.(3)Metabolic stressors such as starvation result in stimulation of AMPK via LKB1.Activation of AMPK preserves TJ assembly,possibly through MRLC,and prevents cell proliferation and protein synthesis through inhibitio
27、n of the mTOR pathway.(4)Binding of TGF to TGFR2 re-sults in recruitment and activation of TGFR1 at both TJs and AJs.At TJs,this interaction recruits Par6 and an E3 ubiquitin ligase,Smurf1,which ubiquitinates RhoA.RhoAs subsequent degradation results in disassembly of TJs and loss of AB polarity.At
28、AJs,TGFR signaling recruits PI3K-Ras-GTPase complex,which results in the phosphorylation and disassembly of-and-catenin complexed with E-cadherin.Disassembly of AJs then ensues.(5)Activation of the oncogene ErbB2/HER2/Neu receptor tyrosine kinase by homodimerization at the basolateral membrane recru
29、its Par6-aPKC without Par3,thereby disrupting AB polarity and preventing apop-tosis.(6)The activation of various growth factor receptor tyrosine kinases results in recruitment and activation of an E3 ubiquitin ligase,Hakai,which ubiquitinates E-cadherin at the AJ and promotes its endocytosis and deg
30、radation,thus destabilizing AJs.Abbreviations-ctn,-catenin;AB polarity,apical-basal polarity;AJ,adherens junction;AMOT,angiomotin;AMPK,AMP-activated protein kinase;Anx-2,annexin-2;APC,ad-enomatosis polyposis coli;aPKC,atypical protein kinase C;-ctn,-catenin;TrCP,beta-transducin repeat-containing pro
31、tein;CD2AP,CD2-associated protein;CDK4,cyclin-dependent kinase 4;CKI,casein kinase I;c-Met,hepatocyte growth factor receptor;EGFR,epidermal growth factor receptor;FAK,focal adhesion kinase;GAP,GTPase-activating protein;GEF,guanine nucleotide exchange factor;GSK3,glycogen synthase kinase 3-beta;hScri
32、b,human homolog of scribble;JAM,junctional adhesion molecule;JRAB,junctional Rab13-binding protein;LEF/TCF,lymphoid enhancer factor-1/T cell factor-1;LIMK2,LIM domain kinase;MAGI,(continued on next page)1118.e2 Cell 133,June 13,2008 2008 Elsevier Inc.DOI 10.1016/j.cell.2008.06.002SnapShot:Tight and
33、Adherens Junction SignalingJennifer L.Harder and Ben MargolisDepartments of Internal Medicine and Biological Chemistry,University of Michigan,Ann Arbor,MI 48109,USAmembrane-associated guanylate kinase protein;MAPs,microtubule-associated proteins;mDia1,mammalian homolog of Drosophila diaphanous 1;mDl
34、g/SAP97,mammalian homolog of discs large;mLgl,mammalian homolog of lethal giant larvae;MPDZ/MUPP1,multiple PDZ domain protein;MRLC,regulatory light chain of nonmuscle myosin II;mTOR,mammalian target of rapamycin;N-WASP,neuronal Wiskott-Aldrich syndrome protein;p120/p120ctn,p120 catenin;mLin-2,mammal
35、ian homolog of Lin-2;PATJ,PALS1-associated tight junction protein;PDGFR,platelet-derived growth factor receptor;PDK1,3-phosphoinositide-dependent protein kinase-1;PI3K,phosphoinositide 3-kinase;PIP2,phosphatidylinositol(3,4)bisphosphate;PIP3,phosphatidylinositol(3,4,5)-trisphosphate;PKA,protein kina
36、se A;PKC,calcium-dependent protein kinase C;PPase,generic protein phosphatase;PTEN,phosphatase and tensin homolog;PTP,protein tyrosine phosphatase;PTP1B,protein tyrosine phosphatase 1B;RTK,generic receptor tyrosine kinase;TGF,transforming growth factor;TGFR;TGF receptor;TJ,tight junction;Tsc,tuberou
37、s sclerosis protein;Ub,ubiquitin;VASP,vasodilator-stimulated phosphoprotein;ZONAB,ZO1associated nucleic acid binding protein.RefeRencesBrembeck,F.H.,Rosrio,M.,and Birchmeier,W.(2006).Balancing cell adhesion and Wnt signaling,the key role of beta-catenin.Curr.Opin.Genet.Dev.16,5159.Forcet,C.,and Bill
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