1、中华中医药杂志(原中国医药学报)2023年2月第38卷第2期 CJTCMP,February 2023,Vol.38,No.2 810 mediated NF-kappa B activation by targeting TAB2 and TAB3 for degradation.Nat Immunol,2008,9(4):369-37718 Zhang P,Cao Y,Chen S,et al.Combination of vinpocetine and dexamethasone alleviates cognitive impairment in nasopharyngeal carc
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9、京 100700）摘要：目的：观察大黄虫丸对博来霉素致肺纤维化大鼠Rho/ROCK信号通路表达情况的影响，探讨其防治特发性肺纤维化（IPF）的作用机制。方法：将80只健康雄性SD大鼠随机分为空白对照组（Control组）、模型组（Model组）、吡非尼酮组（PFD组）、大黄虫丸大剂量组（DHZC-H组）、大黄虫丸中剂量组（DHZC-M组）、大黄虫丸小剂量组（DHZC-L组）。采用一次性气管内滴入博来霉素（5 mg/kg）复制肺纤维化大鼠模型；给药28 d后处死大鼠，行ELISA法检测TGF-1、TNF-含量；RT-PCR法检测ROCK1、-SMA mRNA表达；Western blot法检测p
10、-RhoA、ROCK1、p-MLC蛋白表达。结果：与Model组比较，各治疗组TGF-1浓度和DHZC-L组TNF-浓度显著降低（P0.01，P0.05）；DHZC-L组TGF-1浓度显著低于PFD组（P0.05）。与Model组和PFD组比较，DHZC-M组和DHZC-L组ROCK1、-SMA mRNA相对表达量均显著降低（P0.01，P0.05）。与Model组比较，大黄虫丸各剂量组p-RhoA蛋白相对表达量以及各治疗组ROCK1、p-MLC蛋白相对表达量显著降低（P0.05，P0.01）；且DHZC-L组p-RhoA、ROCK1、p-MLC蛋白相对表达量显著低于PFD组（P0.01）。结
11、论：大黄虫丸可通过下调TGF-1介导的RhoA/ROCK1信号通路及减少-SMA mRNA及TNF-表达，起到抗炎抗纤维化作用。研究报告通信作者：张立山，北京市东城区海运仓5号北京中医药大学东直门医院，邮编：100700，电话：010-84013357，E-mail：内文2.indd 8102023/2/28 10:36:06中华中医药杂志(原中国医药学报)2023年2月第38卷第2期 CJTCMP,February 2023,Vol.38,No.2 811 关键词：大黄虫丸；干血；特发性肺纤维化；-SMA；TGF-1；RhoA/ROCK1；肺络基金资助：国家自然科学基金项目（No.81673
12、904），北京中医药大学东直门医院2020科技创新专项（No.DZMKJCX-2020-025）Effects of Dahuang Zhechong Pills on the expression of Rho/ROCK signal pathway in Bleomycin-induced pulmonary fibrosis in ratsJIANG Feng-li,HUANG Mao,SHI Yu-chen,CAI Song,REN Shi-jie,SUN Wen-li,ZHANG Jing,ZHANG Li-shan(Department of Respiratory Medicin
13、e,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)Abstract:To observe the effects of Dahuang Zhechong Pills(DHZC)on the expression of Rho/ROCK signaling pathway in bleomycin-induced pulmonary fibrosis rats,and to explore its mechanism of prevention and treatment on id
14、iopathic pulmonary fibrosis(IPF).Methods:Eighty healthy male SD rats were randomly divided into a blank control group(Control group),a model group(Model group),a pirfenidone group(PFD group),a large-dose group of DHZC(DHZC-H group),a medium-dose group of DHZC(DHZC-M group),a small-dose group of DHZC
15、(DHZC-L group).Pulmonary fibrosis rat model was replicated with a one-time instillation of bleomycin(5 mg/kg)into the trachea;the rats were sacrificed 28 d after the administration,the TGF-1 and TNF-content was detected by the ELISA method;RT-PCR method was used to detect ROCK1 and-SMA mRNA expressi
16、on;Western blot method was used to detect p-RhoA,ROCK1 and MLCP protein expression.Results:Compared with Model group,TGF-1 concentration in treatment groups and TNF-concentration in DHZC-L group were significantly decreased(P0.01,P0.05);The concentration of TGF-1 in DHZC-L group was significantly lower than that in PFD group(P0.05).Compared with Model group and PFD group,the mRNA relative expression levels of ROCK1 and-SMA in DHZC-M and DHZC-L groups were significantly decreased(P0.01,P0.05).Com