1、Designation:E130213An American National StandardStandard Guide forAcute Animal Toxicity Testing of Water-MiscibleMetalworking Fluids1This standard is issued under the fixed designation E1302;the number immediately following the designation indicates the year oforiginal adoption or,in the case of rev
2、ision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This guide defines acute animal toxicity tests and setsforth the references for procedures to assess the
3、 acute toxicityof water-miscible metalworking fluids as manufactured.1.2 Although water-miscible metalworking fluids are typi-cally used at high dilution,dilution rates vary widely.Additionally,there is potential for exposure to the metalwork-ing fluid as manufactured.1.3This standard does not purpo
4、rt to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2E758 Tes
5、t Method for Mammalian Acute Percutaneous Tox-icity(Withdrawn 2010)3E981 Test Method for Estimating Sensory Irritancy of Air-borne ChemicalsE993 Test Method for Evaluation of Delayed Contact Hy-persensitivity(Withdrawn 2010)3E1103 Test Method for Determining Subchronic DermalToxicity(Withdrawn 2010)
6、3E1542 Terminology Relating to Occupational Health andSafetyE2523 Terminology for Metalworking Fluids and Opera-tions2.2 CPSC Standards:416 CFR Part 150016 CFR Part 1500.316 CFR Part 1500.4016 CFR Part 1500.4116 CFR Part 1500.422.3 DOT Standards:449 CFR Part 173,Appendix A49 CFR Part 173.343a149 CFR
7、 Part 173.343a249 CFR Part 173.343a32.4 EPATSCA Standards:440 CFR 79240 CFR 870.110040 CFR 870.120040 CFR 870.130040 CFR 870.240040 CFR 870.250040 CFR 870.26002.5 OSHA Standards:429 CFR 1910.120029 CFR 1910.1200 Appendix A,3(a)and 6(a)29 CFR 1910.1200 Appendix A,3(b)and 6(b)29 CFR 1910.1200 Appendix
8、 A,3(c)and 6(c)29 CFR 1910.1200 Appendix A,43.Terminology3.1 For definitions of terms in this practice relating totoxicological testing,refer to Terminology E2523.For defini-tions of terms in this practice relating to occupational healthand safety,refer to Terminology E1542.3.2 Definitions of Terms
9、Specific to This Standard:3.2.1 limit test,nan acute toxicity test in which,if noill-effects occur at a pre-selected maximum dose,no furthertestingatgreaterexposurelevelsisrequired.http:/sis.nlm.nih.gov/enviro/iupacglossary/glossaryl.html1This test method is under the jurisdiction of ASTM Committee
10、E34 onOccupational Health and Safety and is the direct responsibility of SubcommitteeE34.50 on Health and Safety Standards for Metal Working Fluids.Current edition approved July 1,2013.Published July 2013.Originally approvedin 1989.Last previous edition approved in 2012 as E1302-12.DOI:10.1520/E1302
11、-13.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is refe
12、renced onwww.astm.org.4Available from Supt.of Documents,U.S.Government Printing Office,Washington,DC 20402.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 4.Significance and
13、 Use4.1 Application of this guide will provide information onthe acute toxicity of water-miscible metalworking fluids andwill assist the user in evaluating the potential health hazards ofthe fluid and developing appropriate work practices.A water-miscible metalworking fluid is a concentrate designed
14、 to bediluted in water for use.4.2 Water-miscible metalworking fluids are complex chemi-cal mixtures.The United States Occupational Safety andHealth Administration(OSHA)Hazard Communication Stan-dard(see A1.8)outlines procedures for the hazard determina-tion of mixtures and states that if a mixture
15、has not been testedas a whole,then the mixture shall be assumed to present thesame hazards as do the components that comprise 1%(byweight or volume)or greater of the mixture,except that themixture shall be assumed to present a carcinogenic hazard if itcontains a component in concentrations of 0.1%or
16、 greater,which is considered to be a carcinogen(as defined in OSHAStandard 29 CFR 1910.1200).The determination of when totest a mixture as a whole and which toxicity tests areappropriate for the product must be made by a healthprofessional,qualified in evaluating toxicological data.4.3 Acute toxicology testing of water-miscible metalwork-ing fluids consists of several individual tests including acuteoral,dermal,or inhalation toxicity,eye irritation,skin irritationor corrosion,or both,skin sensit
