1、Designation:E183913Standard Test Method forEfficacy of Slimicides for the Paper IndustryBacterial andFungal Slime1This standard is issued under the fixed designation E1839;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of l
2、ast revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method presents a procedure to evaluate theefficacy of slimicides for the control of bacterial and fungalslimes in
3、 paper mill systems and their counterparts.1.2 It is the responsibility of the investigator to determinewhether Good Laboratory Practices(GLP)are required and tofollow them where appropriate(40 CFR 160).1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are
4、 included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior
5、 to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE1054 Test Methods for Evaluation of Inactivators of Anti-microbial AgentsE2756 Terminology Relating to Antimicrobial and AntiviralAgents2.2 TAPPI Standard:T 205 Forming Handsheets for Physical Tests of Pulp32.3 C
6、FR Standard:40 CFR Part 160 Good Laboratory Practice Standards43.Terminology3.1 For definitions of terms related to this practice,seeTerminology E2756.3.2 Definitions:3.2.1 furnish,npulp slurry fed to a paper machine.Thetype of pulp(sulfite,Kraft,mechanical),the source of fiber(virgin,recycled inclu
7、ding pre-or post-consumer waste paper),and the pH are used to designate a specific type of furnish.3.2.2 microbicide,na physical or chemical agent that killsmicroorganisms.3.2.3 pulp,nwood separated by chemical or mechanicalmeans into their fibrous components.The pulp is used to makepaper,paper boar
8、d,or pulp sheets after specific treatments.Hardwood pulp is made from trees,such as maples or oaks,andsoftwood pulp is produced from trees,such as pines.3.2.4 pulp slurry,nan aqueous combination of cellulosicfibers,fillers,and other additives used for specific grades ofpaper.3.2.5 slime,nbiofilm.3.2
9、.6 slimicides,nchemical agent added during pulp andpaper processing to reduce the growth of slime-formingmicroorganisms.4.Summary of Test Method4.1 Bacterial cells or fungal spores are added to acid oralkaline pulp slurries,or both,treated with slimicides toachieve final concentrations of 2 106to 1
10、107bacteria/mLand/or 105to 106fungal spores/mL,and incubated at appro-priate temperature for determined time periods.Aliquots of thetest suspension are then neutralized,plated onto bacterial orfungal medium,and observed for growth.Results with biocideare compared to results without biocide(control).
11、4.2 As a performance standard,an effective slimicide is onethat shows a continued reduction in bacterial and fungal countsrelative to the control over the duration of the test.5.Significance and Use5.1 This test method is to be used to determine if a slimecontrol agent has application in the paper i
12、ndustry for controlof bacterial or fungal slime/biofilm.1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agentsand is the direct respon-sibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved April 1,2013.P
13、ublished May 2013.Originallyapproved in 1996.Last previous edition approved in 2007 as E1839 07.DOI:10.1520/E1839-13.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the
14、standards Document Summary page onthe ASTM website.3Available from Technical Association of the Pulp and Paper Industry(TAPPI),15 Technology Parkway South,Norcross,GA 30092,http:/www.tappi.org.4Available from U.S.Government Printing Office Superintendent of Documents,732 N.Capitol St.,NW,Mail Stop:S
15、DE,Washington,DC 20401,http:/www.access.gpo.gov.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.2 This test method is run in acid,alkaline,or acid andalkaline conditions to determine the efficacy of the slimecontrol agent.5.3 The test c
16、onditions may be modified to reflect intendeduse patterns in typical paper mill systems,including use ofactual paper mill furnish.6.Apparatus6.1 Balance:6.1.1 Plant Balance,one sensitive to 0.1 g at load of 200 g,with platform to weigh furnish and bottles used in the test.6.1.2 Analytical Balance,one sensitive to 0.1 mg used forweighing the candidate slime control agent.6.2 Sample Containers(Sterile),120-mL plastic specimencontainers with screw-cap lids are ideal for holding testmaterials.Other
