1、Designation:E264608Standard Specification forAmblyseius cucumeris(=Neoseiulus)Oudemans(Acarina:Phytoseiidae)1This standard is issued under the fixed designation E2646;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last r
2、evision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification includes standard terminology,classification,and referenced documents as well as descriptionof the test method
3、s for determining the number of Amblyseiuscucumeris Oudemans supplied on the bulk carrier or in slowrelease sachets.Description of the method for assessing thepurity of shipments is also included.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are includ
4、ed in thisstandard.2.Referenced Documents2.1 ASTM Standards:2E2200 Specification for Information Included with Packag-ing of Multi-Cellular Biological Control Organisms(Withdrawn 2010)33.Terminology3.1 name of productAmblyseius cucumeris Oudemans.3.2 preferred host preyWestern flower thrips(Franklin
5、-iella occidentalis).3.3 onion thrips(Thrips tabaci)also cyclamen and broadmites.3.4 life stage when shippedall stages,eggs,nymphs,adults.4.Classification4.1 PhylumArthropoda.4.2 ClassArachnida.4.3 OrderAcarina.4.4 FamilyPhytoseiidae.4.5 GenusAmblyseius=Neoseiulus.4.6 Speciescucumeris.5.Summary of T
6、est Method(Determining the Number ofN.cucumeris Released from a Slow Release Sachet)5.1 The test describes methods for(1)estimation of thenumber of A.cucumeris released from sachet based on exam-ining a few randomly selected sachets per shipment,and(2)assessing the purity of shipment.5.2 Afew sachet
7、s are chosen randomly from a shipment andeach sachet is suspended from a wire hanger and placed on asticky trap surface(“Catch it,”Silvandersson Sweden AB).Mites are allowed to disperse from sachets,and each week,mites caught on the sticky trap are counted and recorded.6.Significance and Use6.1 The
8、biological control of flower thrips by N.cucumerisis,in part,dependent on accurate release rates and consistentrelease pattern of N.cucumeris.Supplying fewer predatorymites than indicated on the label or restricting duration ofrelease may upset host-predator balance and could lead to thefailure of b
9、iological control.This test was developed for thebenefit of biocontrol producers and IPM practitioners.7.Materials7.1 Yellow sticky traps with a grid and coated with dry glue.7.2 A 20-cm high wire hanger.8.Test Unit8.1 A shipment of A.cucumeris is considered a test unit.9.Pre-Test Conditions9.1 Samp
10、les could be held in a cool place at 10 to 20C outof direct sunlight for a maximum of 24 h before testing.10.Sample Size10.1 Choose four or more sachets from a shipment(needs tobe determined).1This specification is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternat
11、ive Control Agents and is the directresponsibility of Subcommittee E35.30 on Natural Multi-Cellular(Metazoan)Biological Control Organisms.Current edition approved Oct.1,2008.Published October 2008.DOI:10.1520/E2646-08.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM
12、Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,W
13、est Conshohocken,PA 19428-2959.United States1 11.Testing and Calculations11.1 Suspend each sachet on a wire hanger and place on asticky trap surface,one per trap(Fig.1).Laboratory evaluationshould be carried out under 16:8 D:N photoperiod 18 to 21Cand 75 to 80%RH(relative humidity).When testing is c
14、arriedout in the greenhouse,place the sticky traps and sachet insidea small cage(45 by 30 by 27 cm)made of a white breathablefabric(Fig.2).Position the cage within the crop but away fromthe heating pipes.11.2 Allow mites to disperse until from the sachet until nomore mites are released;that is,six o
15、r more weeks.Change thesticky traps weekly and count and record the number of N.cucumeris caught on the trap.Calculate the average number ofthe predatory mites released per sachet12.Results12.1 The average number of A.cucumeris released persachet should be equal to or greater than the number specifi
16、edon the package.13.Summary of Test Method(Determining the Purityand Number of Live Adults and Nymphs in 1.5-LContainers with Amblyseius cucumeris)13.1 This test describes a method of estimating the numberof A.cucumeris in a standard 1.5-L package with bran carrier,based on a random sampling and counts of live nymph andadult predators.14.Significance and Use14.1 The biological control of flower thrips by A.cucumerisdepends on accurate release numbers of predators and absenceof other harmful inse
