1、Designation:E219810Standard Specification forPhytoseiulus persimilis Athias-Henriot(Acarina:Phytoseiidae)1This standard is issued under the fixed designation E2198;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revi
2、sion.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification includes standard terminology,clas-sification,and referenced documents as well as description ofthe test method d
3、etermining whether the number of P.persi-milis in the shipment and the purity of shipments meet thequantity specification.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.2.Terminology2.1 Definitions of Terms Specific to This
4、Standard:2.1.1 bulk carrierincludes vermiculite,corn grits,orwood shavings.2.1.2 carriervermiculite,corn grits,wood shavings,orbean leaves.2.1.3 critical valuea number that the test statistics iscompared to in order to determine whether the quantityrequirement has been met.Critical values depend on
5、theprobability of error that can be tolerated and on the number ofsamples used in the test.2.1.4 life stage when shippedadults and immature.2.1.5 name of productPhytoseiulus persimilis Athias-Henriot2.1.6 package claimthe number of adults expected in eachcontainer.2.1.7 preferred host preytwo-spotte
6、d spider mite,Tetrany-chus urticae Koch2.1.8 test statisticsthe average number of adults in asample.3.Classification3.1 PhylumArthropoda.3.2 ClassArachnida.3.3 OrderAcarina.3.4 FamilyPhytoseiidae.3.5 GenusPhytoseiulus.3.6 Speciespersimilis.Test Method for Determining the Number of Live P.persimilis
7、per Shipment Supplied on a Bulk Carrier andAssessment of Purity of Shipments4.Scope4.1 The test describes the method for determining if thenumber of the predatory mites conforms to the advertisedstandard and the method for assessment of purity of shipments.5.Summary of Test Method5.1 The carrier wit
8、h the predatory mites is mixed thoroughlyand a few random samples are taken,one per container.Measurements may be done either by weighing,or by measur-ing volume.The size of each sample is scaled to maintain thesame proportion(2%)of the sample to the total.The predatorymites running out of or stayin
9、g in the carrier are counted andrecorded.Results are used to calculate the mean number ofmites running out of the carrier.The mean is the knownattribute of the assessment method and is used to judge whetherthe specification is met.In each container,possible livecontaminants will be identified and re
10、corded.6.Significance and Use6.1 This method was developed to determine that thenumber of P.persimilis supplied in the shipment meets thepackage claim.The application of this method will ensure astandardized evaluation of the product and a judicious decisionabout product compliance to the package cl
11、aim.7.Materials7.1 Non-toxic white glue or dish detergent,light source,scale(0.01 gr minimum),mixing container,and magnifier(10).8.Test Unit8.1 A single shipment with P.persimilis is considered a testunit.1This specification is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials
12、,and Alternative Control Agents and are the directresponsibility of Subcommittee E35.30 on Natural Multi-Cellular(Metazoan)Biological Control Organisms.Current edition approved Oct.1,2010.Published November 2010.Originallyapproved in 2002.Last previous edition approved in 2002 as E2198 02.DOI:10.152
13、0/E2198-10.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 9.Pre-Test Conditions9.1 Store the containers with P.persimilis between 10 and12C,RH 60 6 5%,for a minimum of 1 h and maximum of24 h prior to examination.10.Sampling10.1 Select fo
14、ur or more samples(bottles)per shipment.11.Mixing and Weight Recording11.1 Record the weight of the container with carrier andpredators.Rotate the contents of the container gently until allpredators are removed from the container wall and mixed wellwith carrier.Pour half of the carrier with predator
15、s into amixing container.Mixing container should be approximatelyfour times larger than container with P.persimilis.Mix theremaining contents for a few seconds and empty into themixing container.Rotate carrier with predators gently forapproximately 15 s so that carrier always touches containerwalls.
16、Record the weight of empty container and determinetotal weight of carrier and predators.Take one sample perbottle each consisting of 2%of carrier weight or volume for200 to 500 mL containers,or minimum 5%of carrier weightor volume for 200 mL containers.12.Counting Arena and Test Conditions12.1 Pour a thin line of a white glue or liquid detergent toform a circle on a white sheet of paper and spread each sampleinside the circle.This will prevent predators from escapingduring counting.Place the sam
