1、Designation:D839922Standard Test Method forMulti-residue Analysis of Pesticides in Dried Cannabis andHemp Raw Materials Using Liquid Chromatography-TandemMass Spectrometry(LC-MS/MS)1This standard is issued under the fixed designation D8399;the number immediately following the designation indicates t
2、he year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method allows for the concentration determi-na
3、tion of the pesticides listed in Table 1 and shall apply to anydried raw material from a cannabis plant(Note 1,Note 2)regardless of the type of cannabis plant from which it wasderived(1,2).2For the sake of brevity,the term“cannabis”shall be used from now on to refer to any type of cannabis plantincl
4、uding those which can be classified as hemp.The proce-dure includes sub-sampling a ground,homogenous sample,liquid-solid extraction with acetonitrile:acetic acid(100:1,v:v),solid phase extraction with C18 SPE media,dilution in 3 mMammonium formate in water with 0.1%formic acid and 3 mMammonium forma
5、te in methanol with 0.02%formic acid in a20:80 ratio(v:v)and analysis by LC-MS/MS.This procedureencompasses the entire process from sample preparation toanalyte quantitation encompassing a range of 0.005 g g to0.500 gg.NOTE1For this test method,dried raw material from a cannabis plantincludes one or
6、 more of inflorescence,leaves,or stems.NOTE2Certain jurisdictions or regulations may require specific partsof the plant to be included or excluded for analysis and those regulationswill take precedence for the selection of plant parts.1.2 UnitsThe values stated in SI units are to be regardedas stand
7、ard.No other units of measurement are included in thisstandard.1.3 Table 1 lists the analytes measured by this test method.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-pr
8、iate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopm
9、ent of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:3D1193 Specification for Reagent WaterD8245 Guide for Disposal of Resin-Containing CannabisRaw Materials and Downstream
10、ProductsD8270 Terminology Relating to CannabisD8282 Practice for Laboratory Test Method Validation andMethod Development3.Terminology3.1 DefinitionsFor general terms related to cannabis,referto Terminology D8270.3.2 Definitions of Terms Specific to This Standard:3.2.1 blank,na cannabis-only sample p
11、repared from thesame homogenous cannabis blend used to prepare the calibra-tion curve,without the addition of internal standard workingsolution(ISWS).3.2.2 blank-0,na cannabis-only sample prepared from thesame homogenous cannabis blend used to prepare the calibra-tion curve,with the addition of ISWS
12、.3.2.3 blank-S,na solvent blank prepared from only themobile phases,without cannabis or analytes.3.2.4 growth regulator,na class of chemical compoundsused to modulate the growth and development of a crop.3.2.5 pesticide,na product or substance used as a meansto reduce or mitigate pests such as insec
13、ts,fungus,bacteria,and weeds.3.2.5.1 DiscussionMay be used in gardens,crops,wood-lands or recreational areas,these are fungicides,insecticides,herbicides,and biocontrols approved for use by regulatoryauthorities to control pests harmful to plants,humans,oranimals.1This test method is under the juris
14、diction of ASTM Committee D37 onCannabis and is the direct responsibility of Subcommittee D37.03 on Laboratory.Current edition approved July 1,2022.Published August 2022.DOI:10.1520/D8399-22.2The boldface numbers in parentheses refer to the list of references at the end ofthis standard.3For referenc
15、ed ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocke
16、n,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.13.3 Abbreviated Terms,Acronyms,and Initialisms3.3.1 HPLC,nhigh performance liquid chromatography3.3.2 ICS,nindependent check standard3.3.3 ISWS,ninternal st
