1、Designation:D613922Standard Test Method forDetermining the Aerobic Aquatic Biodegradation ofLubricants or Their Components Using the Gledhill ShakeFlask1This standard is issued under the fixed designation D6139;the number immediately following the designation indicates the year oforiginal adoption o
2、r,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This test method covers the determination of the degreeof aerobic aquatic biodegrada
3、tion of fully formulated lubri-cants or their components on exposure to an inoculum undercontrolled laboratory conditions.This test method is an ulti-mate biodegradation test that measures carbon dioxide(CO2)evolution.1.2 This test method is intended to specifically address thedifficulties associate
4、d with testing water insoluble materialsand complex mixtures such as are found in many lubricants.1.3 This test method is designed to be applicable to allnon-volatile lubricants or lubricant components that are nottoxic and not inhibitory at the test concentration to theorganisms present in the inoc
5、ulum.1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish app
6、ro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.Specific hazards are discussed in Section 10.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization establi
7、shed in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterD1293 Test Methods for pH of Wat
8、erD4447 Guide for Disposal of Laboratory Chemicals andSamplesD5291 Test Methods for Instrumental Determination ofCarbon,Hydrogen,and Nitrogen in Petroleum Productsand LubricantsD5864 Test Method for Determining Aerobic Aquatic Bio-degradation of Lubricants or Their ComponentsE943 Terminology Relatin
9、g to Biological Effects and Envi-ronmental Fate2.2 ISO Standard:34259:1992(E)Petroleum ProductsDetermination and ap-plication of precision data in relation to methods of test2.3 APHA Standards:42540B Total Solids Dried at 103105C9215 Heterotrophic Plate Count3.Terminology3.1 Definitions:3.1.1 Defini
10、tions of terms applicable to this test method thatare not described herein appear in theASTM Online Dictionaryof Engineering Science and Technology5or TerminologyE943.1This test method is under the jurisdiction of ASTM Committee D02 onPetroleum Products,Liquid Fuels,and Lubricants and is the direct
11、responsibility ofSubcommittee D02.12 on Environmental Standards for Lubricants.Current edition approved April 1,2022.Published April 2022.Originallyapproved in 1997.Last previous edition approved in 2018 as D6139 18.DOI:10.1520/D6139-22.2For referenced ASTM standards,visit the ASTM website,www.astm.
12、org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from American National Standards Institute(ANSI),25 W.43rd St.,4th Floor,New York,NY 10036,http:/www.ansi.org.4Methods
13、 from Standard Methods for the Examination of Water and Wastewater,latest edition.Available from the American Public Health Association(APHA),800I Street,NW,Washington,DC 20001.5ASTMOnlineDictionaryofEngineeringScienceandTechnology(Stock#DEFONLINE)is available on theASTM website,www.astm.org,or cont
14、actASTM Customer Service at serviceastm.org.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recogn
15、ized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.13.1.2 activated sludge,nthe precipitated solid matter,consistingmain
16、lyofbacteriaandotheraquaticmicroorganisms,that is produced at a domestic wastewatertreatment plant and is used primarily in secondary sewagetreatment to microbially oxidize dissolved organic matter in theeffluent.3.1.3 aerobic,adj(1)taking place in the presence ofoxygen;(2)living or active in the presence of oxygen.3.1.4 biodegradation,nthe process of chemical break-down or transformation of a material caused by organisms ortheir enzymes.3.1.4.1 DiscussionBiodegradation is only one mechanismby w
