1、Designation:D673101(Reapproved 2011)Standard Test Method forDetermining the Aerobic,Aquatic Biodegradability ofLubricants or Lubricant Components in a ClosedRespirometer1This standard is issued under the fixed designation D6731;the number immediately following the designation indicates the year ofor
2、iginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers a procedure for determining thedegree of bio
3、degradability of lubricants or their components inan aerobic aqueous medium on exposure to an inoculum undercontrolled laboratory conditions.This test method is an ulti-mate biodegradation test that measures oxygen demand in aclosed respirometer.1.2 This test method is suitable for evaluating the bi
4、odeg-radation of volatile as well as nonvolatile lubricants or lubri-cant components.1.3 This test method is applicable to lubricants and lubricantcomponents which are not toxic and not inhibitory to the testmicroorganisms at the test concentration.1.4 The values stated in SI units are to be regarde
5、d asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the
6、applica-bility of regulatory limitations prior to use.Specific hazards aregiven in Section 10.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD1293 Test Methods for pH of WaterD4175 Terminology Relating to Petroleum,PetroleumProducts,
7、and LubricantsD4447 Guide for Disposal of Laboratory Chemicals andSamplesD6384 Terminology Relating to Biodegradability and Eco-toxicity of LubricantsE943 Terminology Relating to Biological Effects and Envi-ronmental Fate2.2 ISO Standards:3ISO 4259:1992(E)Petroleum ProductsDetermination andApplicati
8、on of Precision Data in Relation to Methods ofTestISO 6107-2:1997 Water QualityVocabularyPart 2ISO 8192:1986 Water QualityTest for Inhibition of OxygenConsumption by Activated SludgeISO 9408:1999 Water QualityEvaluation of Ultimate Aero-bic Biodegradability of Organic Compounds in AqueousMedium by D
9、etermination of Oxygen Demand in aClosed Respirometer2.3 OECD Standards:4OECD 301F:1992 Ready Biodegradability-Manometric Re-spirometry2.4 APHA Standards:52540B Total Solids Dried at 103-105C9215 Heterotrophic Plate Count3.Terminology3.1 Definitions:3.1.1 Definitions of terms applicable to this test
10、 methodappear in the Compilation of ASTM Standard Definitions andthe following terminology standards:D1129,D4175,D6384,E943,and ISO 6107-2:1997.3.1.2 activated sludge,nthe precipitated solid matter,consistingmainlyofbacteriaandotheraquaticmicroorganisms,that is produced at a domestic wastewater1This
11、 test method is under the jurisdiction of ASTM Committee D02 onPetroleum Products,Liquid Fuels,and Lubricantsand is the direct responsibility ofSubcommittee D02.12 on Environmental Standards for Lubricants.Current edition approved May 1,2011.Published May 2011.Originallyapproved in 2001.Last previou
12、s edition approved in 2005 as D673101(2005).DOI:10.1520/D6731-01R11.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM websit
13、e.3Available from American National Standards Institute(ANSI),25 W.43rd St.,4th Floor,New York,NY 10036,http:/www.ansi.org.4Available from Organisation for Economic Cooperation and Development(OECD),2 rue Andr Pascal,F-75775,Paris Cedex 16,France,http:/www.oecd.org.5From Standard Methods for the Exa
14、mination of Water and Wastewater,latestedition.Available from the American Public Health Assoc.,1015 18th St.,NW,Washington,DC 20036.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 treatment plant and is used primarily in secondary sewage
15、treatment to microbially oxidize dissolved organic matter in theeffluent.3.1.3 aerobic,adj(a)taking place in the presence ofoxygen;(b)living or active in the presence of oxygen.3.1.4 biochemical oxygen demand(BOD),nthe massconcentration of dissolved oxygen consumed under specifiedconditions by the b
16、iological oxidation of organic or inorganicmatter,or both.3.1.4.1 DiscussionBOD determination is performed usingempirical tests employing standardized laboratory procedures.These tests measure oxygen utilization during a specifiedincubation period for the biochemical degradation of organicmaterial(carbonaceous demand)in water.3.1.5 biodegradation,nthe process of chemical break-down or transformation of a test material caused by microor-ganisms or their enzymes.3.1.5.1 DiscussionBiodegradation is
