1、Designation:D558919Standard Test Method forDetermining the Resistance of Paint Films and RelatedCoatings to Algal Defacement1This standard is issued under the fixed designation D5589;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,th
2、e year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers an accelerated method fordetermining the relative resistance of a paint or coating film t
3、oalgal growth.NOTE1It is hoped that a ranking of relative performance would besimilar to that ranked from outdoor exposures.However,this test methodshould not be used as a replacement for exterior exposure since manyother factors,only a few of which are listed will affect those results.NOTE2ASTM wea
4、thering standards are no longer referenced in thisdocument,but Practices D822,D4141,D4587,D5031,and D6695 arecommonly used.1.2 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.3 This standard does not purport to address all of
5、thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accor-da
6、nce with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standa
7、rds:2D822 Practice for Filtered Open-Flame Carbon-Arc Expo-sures of Paint and Related CoatingsD4141 Practice for Conducting Black Box and Solar Con-centrating Exposures of CoatingsD4587 Practice for Fluorescent UV-Condensation Expo-sures of Paint and Related CoatingsD5031 Practice for Enclosed Carbo
8、n-Arc Exposure Tests ofPaint and Related CoatingsD6695 Practice for Xenon-Arc Exposures of Paint andRelated Coatings3.Summary of Test Method3.1 This test method outlines a procedure to(1)prepare asuitable specimen for testing,(2)inoculate the specimen witha mixture of the proper algal species,(3)exp
9、ose the inoculatedsamples under the appropriate conditions for growth,and(4)provide a schedule and guidelines for visual growth ratings.This test method is not designed to include all the necessaryprocedures to maintain the proper microbiological techniquesrequired to provide the most accurate resul
10、ts.4.Significance and Use4.1 Defacement of paint and coating films by algal growth isa common phenomenon under certain conditions.It is gener-ally known that differences in the environment,lighting,temperature,substrate,and other factors in addition to thecoating composition affect the susceptibilit
11、y of a given paintedsurface.This test method attempts to provide a means tocomparatively evaluate different coating formulations for theirrelative performance under a given set of conditions.It doesnot imply that a coating that resists growth under theseconditions will necessarily resist growth in t
12、he actual applica-tion.4.2 Familiarity with microbiological techniques is required.This test method should not be used by persons without at leastbasic microbiological training.5.Apparatus and Materials5.1 Balance,capable of weighing to 0.10 g.5.2 Incubator,or other device capable of maintaining aco
13、nstant temperature between 25 6 2C,relative humidity of85%,and having a constant full spectrum(see Note 3)lightsource.5.3 Refrigerator.5.4 Petri Dishes,100 by 15 mm(3.9 by 0.6 in.).1This test method is under the jurisdiction of ASTM Committee D01 on Paintand Related Coatings,Materials,and Applicatio
14、ns and is the direct responsibility ofSubcommittee D01.28 on Biodeterioration.Current edition approved Dec.1,2019.Published December 2019.Originallyapproved in 1994.Last previous edition approved in 2013 as D5589 09(2013).DOI:10.1520/D5589-19.2For referenced ASTM standards,visit the ASTM website,www
15、.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis internatio
16、nal standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.5.5 Autoclave.5.6 Paint Brush,coarse bristle,12 to 19 mm(12to34in.).5.7 Test Substrate,filter paper,either regular paper or glassfiber,approximately 4.2 cm(1.65 in.)in diameter,or draw-down paper(unlac
