1、Designation:D476306(Reapproved 2020)Standard Practice forIdentification of Chemicals in Water by FluorescenceSpectroscopy1This standard is issued under the fixed designation D4763;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the y
2、ear of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice allows for the identification of 90 chemi-cals that may be found in water or in surface layers on wate
3、r.This practice is based on the use of room-temperature fluores-cence spectra taken from lists developed by the U.S.Environ-mental Protection Agency and the U.S.Coast Guard(1).Ref(1)is the primary source for these spectra.This practice is alsobased on the assumption that such chemicals are either pr
4、esentin aqueous solution or are extracted from water into anappropriate solvent.21.2 Although many organic chemicals containing aromaticrings,heterocyclic rings,or extended conjugated double-bondsystems have appreciable quantum yields of fluorescence,thispractice is designed only for the specific co
5、mpounds listed.Ifpresent in complex mixtures,preseparation by high-performanceliquidchromatography(HPLC),columnchromatography,or thin-layer chromatography(TLC)wouldprobably be required.1.3 If used with HPLC,this practice could be used for theidentification of fluorescence spectra generated by optica
6、lmultichannel analyzers(OMA)or diode-array detectors.1.4 For simple mixtures,or in the presence of other non-fluorescing chemicals,separatory techniques might not berequired.The excitation and emission maximum wavelengthslisted in this practice could be used with standard fluorescencetechniques(see
7、Refs(2-6)to quantitate these ninety chemicalsonce identification had been established.For such uses,gen-eration of a calibration curve,to determine the linear range foruse of fluorescence quantitation would be required for eachchemical.Examination of solvent blanks to subtract or elimi-nate any fluo
8、rescence background would probably be required.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicabil
9、ity of regulatory limitations prior to use.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the Wor
10、ld Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:3D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterE131 Terminology Relating to Molecular SpectroscopyE275 Practice for Describing and Measuring Performance ofUltraviolet and
11、 Visible Spectrophotometers3.Terminology3.1 DefinitionsFor definitions of terms used in thispractice,refer to Terminology D1129,Specification D1193,and definitions under the jurisdiction of Committee E13 suchas Terminology E131 and Practice E275.4.Summary of Practice4.1 This practice uses well teste
12、d fluorescence techniques todetect and identify(or determine the absence of)90 chemicalsthat have relatively high fluorescence yields.Table 1 lists foreach chemical an appropriate solvent(either cyclohexane,water,methyl or ethyl alcohol,depending on solubility),asuggested excitation wavelength for m
13、aximum sensitivity,awavelength corresponding to the emission maximum,thenumber of fluorescence peaks and shoulders,the width(fullwidth at half of the maximum emission intensity)of thestrongest fluorescence peak and the detection limit for theexperimental conditions given.Detection limits could below
14、ered,following identification,by using broader slit widths.1This practice is under the jurisdiction of ASTM Committee D19 on Water andis the direct responsibility of Subcommittee D19.06 on Methods for Analysis forOrganic Substances in Water.Current edition approved Dec.15,2020.Published December 202
15、0.Originallyapproved in 1988.Last previous edition approved in 2012 as D4763 06(2012).DOI:10.1520/D4763-06R20.2The boldface numbers in parentheses refer to a list of references at the end ofthis standard.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Servi
16、ce at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World T
