收藏 分享(赏)

ASTM_E_2896_-_12.pdf

上传人:益****师 文档编号:188356 上传时间:2023-03-04 格式:PDF 页数:14 大小:337.43KB
下载 相关 举报
ASTM_E_2896_-_12.pdf_第1页
第1页 / 共14页
ASTM_E_2896_-_12.pdf_第2页
第2页 / 共14页
ASTM_E_2896_-_12.pdf_第3页
第3页 / 共14页
ASTM_E_2896_-_12.pdf_第4页
第4页 / 共14页
ASTM_E_2896_-_12.pdf_第5页
第5页 / 共14页
ASTM_E_2896_-_12.pdf_第6页
第6页 / 共14页
亲,该文档总共14页,到这儿已超出免费预览范围,如果喜欢就下载吧!
资源描述

1、Designation:E289612Standard Test Method forQuantitative Petri Plate Method(QPM)for Determining theEffectiveness of Antimicrobial Towelettes1This standard is issued under the fixed designation E2896;the number immediately following the designation indicates the year oforiginal adoption or,in the case

2、 of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.INTRODUCTIONThis test method provides a standardized approach to quantitatively determine the effectiveness o

3、fantimicrobial towelettes(wipes)in treating hard non-porous surfaces contaminated with Staphylo-coccus aureus,Pseudomonas aeruginosa,and Salmonella enterica.This test method addresses theneed for a user-friendly,relevant,and reproducible procedure.21.Scope1.1 This test method provides detailed instr

4、uctions forperforming a quantitative evaluation of antimicrobial efficacyof a towelette when challenged against Staphylococcus aureus,Pseudomonas aeruginosa and Salmonella enterica.Themethod may be used with other microbial strains,thoughmodification may be necessary to accommodate recovery.1.1.1 An

5、timicrobial towelettes,designed to decontaminatehard,non-porous surfaces,are diverse in size,matrixcomposition,and packaging.1.1.2 Antimicrobial towelettes also vary in label claims anduse directions.1.2 This quantitative method does not differentiate betweenmechanical removal of inoculum from a sur

6、face and chemicalinactivation of the test microbe;rather,product efficacy isconsidered a combination of both attributes of a towelette-based formulation.1.3 It is the responsibility of the investigator to determinewhether Good Laboratory Practices(GLP Standards40 CFR,Part 160 of FIFRA)are required a

7、nd to follow them whenappropriate.1.4 This standard may involve the use of hazardousmaterials,chemicals and infectious microorganisms and shouldbe performed only by persons with formal training in micro-biology.1.5 Strict adherence to the protocol is necessary for thevalidity of the test results.1.6

8、 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.7 This standard does not address specific product perfor-mance standards established by regulatory authorities;seeSection 10,Note 2 for details.1.8 This standard does not purport

9、 to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 Other Documents:AOAC Offic

10、ial Method 961.02,Germicidal Spray Productsas Disinfectants.Revised 20123AOAC Official Method 955.15,Use-Dilution Method forTesting Disinfectants against Staphylococcus aureus.Re-vised 20123AOAC Official Method 964.02,Use-Dilution Method forTesting Disinfectants against Pseudomonas aeruginosa.Revise

11、d 20123AOAC Official Methods 955.14,Use-Dilution Method forTesting Disinfectants against Salmonella enterica.Re-vised 2012340 CFR,Part 160 Federal Insecticide,Fungicide and Roden-ticideAct(FIFRA);Good Laboratory Practice Standards41This test method is under the jurisdiction of ASTM Committee E35 onP

12、esticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved Nov.15,2012.Published December 2012.DOI:10.1520/E2896-12.2Samalot-Freire,L.,Tomasino,S.F.,and Hasan,J.A.,The Quantitative PetriPlate Method(

13、QPM):A New Method for Assessing the Efficacy of AntimicrobialTowelettes,Presented at the 125thAnnual Meeting of theAOAC International,NewOrleans,LA,2011.3Available from AOAC International,481 North Frederick Ave.,Suite 500,Gaithersburg,Maryland 20877-2417,http:/www.aoac.org.4Available from U.S.Gover

14、nment Printing Office Superintendent of Documents,732 N.Capitol St.,NW,Mail Stop:SDE,Washington,DC 20401,http:/www.access.gpo.gov.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.Terminology3.1 Definitions:3.1.1 antimicrobial towelette(w

15、ipe),na piece of porousmaterial soaked in an antimicrobial liquid that is meant fordecontamination of hard non-porous environmental surfaces bywiping.3.1.2 dilution blank,ntubes of phosphate buffered saline(PBS)or phosphate buffered dilution water(PBDW)or similarinert phosphate buffer solution.3.1.3

16、 carriers,nglass petri plates(150 by 20 mm).3.1.4 colony forming units(CFU),nnumber of microor-ganisms that form colonies(clusters of microorganisms visiblygrowing on the surface of a membrane filter)as a means ofenumerating the total number of viable microorganisms in asample.3.1.5 quality control(QC),nthe procedures,products orservices that meet a laboratorys specified standards of quality.4.Summary of Test Method4.1 This test method provides detailed instructions forperforming a quantitative

展开阅读全文
相关资源
猜你喜欢
相关搜索

当前位置:首页 > 专业资料 > 国外标准

copyright@ 2008-2023 wnwk.com网站版权所有

经营许可证编号:浙ICP备2024059924号-2