1、See online version for legend and references.646 Cell 142,August 20,2010 2010 Elsevier Inc.DOI 10.1016/j.cell.2010.08.006SnapShot:Homologous Recombination in DNA Double-Strand Break RepairGerard Mazn,Eleni P.Mimitou,and Lorraine S.SymingtonDepartment of Microbiology and Immunology,Columbia Universit
2、y Medical Center,New York,NY 10032,USASee online version for legend and references.142,August 20,2010 2010 Elsevier Inc.DOI 10.1016/j.cell.2010.08.006Gerard Mazn,Eleni P.Mimitou,and Lorraine S.SymingtonDepartment of Microbiology and Immunology,Columbia University Medical Center,New York,NY 10032,USA
3、SnapShot:Homologous Recombination in DNA Double-Strand Break RepairGerard Mazn,Eleni P.Mimitou,and Lorraine S.SymingtonDepartment of Microbiology and Immunology,Columbia University Medical Center,New York,NY 10032,USA646.e1 Cell 142,August 20,2010 2010 Elsevier Inc.DOI 10.1016/j.cell.2010.08.006 Hom
4、ologous recombination(HR)provides an important mechanism to repair both accidental and programmed DNA double-strand breaks(DSBs)during mitosis and meiosis.Defects in HR are associated with mutagenesis and predispose to cancer,highlighting the importance of this pathway for preserving genome integrit
5、y(Moynahan and Jasin,2010).HR is active in the S and G2 phases of the cell cycle where it promotes repair of a broken chromatid from an intact sister chromatid,ensuring error-free repair.The DNA transactions associated with HR are accompanied by modifications to histones,most notably phosphorylation
6、 of H2A/H2AX and chromatin remodeling.This SnapShot shows the yeast proteins directly involved in mitotic DSB repair;their mammalian counterparts are shown on the right.The central reaction in homologous recombination is the pairing and exchange of strands between two homologous DNA molecules.This s
7、tep is catalyzed by the conserved Rad51/RecA family of proteins(Chen et al.,2008;San Filippo et al.,2008).To generate the substrate for binding of Rad51,DNA ends are processed to yield 3 single-stranded DNA tails(53 resection).End resection occurs through a biphasic mechanism:first,the Mre11-Rad50-X
8、rs2(MRX)complex and Sae2 clip 50100 nucleotides from the 5 ends of DNA,and then Exo1 or Sgs1-Top3-Rmi1 and Dna2 process the early intermediate to form extensive regions of single-stranded DNA(Mimitou and Symington,2009).Replica-tion protein A(RPA)binds to the single-stranded DNA tails but is displac
9、ed by Rad51 aided by the mediator proteins Rad52 or BRCA2(Rad51 filament formation)(San Filippo et al.,2008).The Rad51 paralogs in the budding yeast Saccharomyces cerevisiae,Rad55 and Rad57,are thought to extend or stabilize the Rad51 nucleoprotein filament(San Filippo et al.,2008).Once formed,the c
10、omplex of Rad51 and single-stranded DNA searches for a homologous sequence in double-stranded DNA and then promotes invasion of the single-stranded DNA into donor double-stranded DNA to form a joint molecule with a displaced strand(D loop).Homologous pairing by Rad51 is stimulated by Rad54,a member
11、of the Swi2/Snf2 family of chromatin remodeling proteins/ATPases(San Filippo et al.,2008).Rad54 displaces Rad51 from double-stranded DNA in vitro,and this could be important for turnover of Rad51,or for uncovering the 3 end of paired intermediates to allow initiation of DNA synthesis(San Filippo et
12、al.,2008).DNA polymerase extends the 3 end from the broken chromosome using the donor strand as a template and replacing nucleotides lost by end resection(Li et al.,2009).To resolve the intermedi-ate by synthesis-dependent strand annealing,the invading strand that has been extended by DNA synthesis
13、is displaced(strand displacement)and anneals to complementary sequences exposed by 53 resection of the other side of the break forming noncrossover products exclusively(strand annealing).In addition to preventing initiation of inap-propriate recombination events by disrupting Rad51 nucleoprotein fil
14、aments,the Srs2 DNA helicase can disrupt D loop intermediates to promote noncrossover recombination(Dupaigne et al.,2008;San Filippo et al.,2008).The Mph1 and RTEL1 helicases also dissociate D loop intermediates to facilitate synthesis-dependent strand annealing(Barber et al.,2008;Prakash et al.,200
15、9).In the canonical DNA double-strand break repair model,the other end of the break interacts with the displaced strand of the strand invasion intermediate(second end cap-ture)and the 3 end primes DNA synthesis,forming a double Holliday junction(dHJ)intermediate(double Holliday junction formation).T
16、he dHJ intermediates can be dissolved or resolved to yield separate intact duplex molecules.Alternatively,the extended D loop structure could be cleaved by the Mus81-Mms4 nuclease prior to formation of a mature dHJ intermediate(early D loop cleavage).Mus81-Mms4 exhibits higher activity toward D loop
17、 and nicked Holliday junction intermediates than intact Holliday junctions and could promote crossovers by cleaving the strand invasion intermediate directly.Dissolution of dHJ intermediates requires the combined activity of the BLM/Sgs1 helicase,which drives migration of the constrained Holliday ju
18、nctions,and the Top3-Rmi1 complex,which decatenates the interlinked strands between the two Holliday junctions eventually leading to noncrossover products(Wu and Hickson,2003).On the other hand,resolution through nucleolytic cleavage of the Holliday junctions can yield crossover(cutting inner strand
19、s of one Holliday junction and outer strands of the other)or noncrossover(cutting both junctions in the same plane)products.To date,four resolvases have been determined to cleave Holliday junction intermediates in vitro:Mus81-Mms4,Slx1-Slx4,Yen1,and Rad1-Rad10(Svendsen and Harper,2010).Factors gover
20、ning the choice of a resolvase are not yet clear,and a given species may preferentially use one of these resolvases in meiosis.For example,Mus81-Mms4 plays an essential role in the resolution of meiotic recombi-nation intermediates in the fission yeast Schizosaccharomyces pombe,whereas SLX4/MUS312 i
21、s the central player in the fruit fly Drosophila melanogaster and the nematode Caenorhabditis elegans(Svendsen and Harper,2010).Following cleavage of Holliday junctions,the ends are ligated to complete the reaction.AcknowledgmentsWe thank W.K.Holloman and H.L.Klein for helpful comments.We are suppor
22、ted by NIH grant GM041784.RefeRencesBarber,L.J.,Youds,J.L.,Ward,J.D.,McIlwraith,M.J.,ONeil,N.J.,Petalcorin,M.I.,Martin,J.S.,Collis,S.J.,Cantor,S.B.,Auclair,M.,et al.(2008).RTEL1 maintains genomic stability by suppressing homologous recombination.Cell 135,261271.Chen,Z.,Yang,H.,and Pavletich,N.P.(200
23、8).Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures.Nature 453,489494.Dupaigne,P.,Le Breton,C.,Fabre,F.,Gangloff,S.,Le Cam,E.,and Veaute,X.(2008).The Srs2 helicase activity is stimulated by Rad51 filaments on dsDNA:Implications for crossover incidence during mitotic recombi
24、nation.Mol.Cell 29,243254.Li,X.,Stith,C.M.,Burgers,P.M.,and Heyer,W.D.(2009).PCNA is required for initiation of recombination-associated DNA synthesis by DNA polymerase delta.Mol.Cell 36,704713.Mimitou,E.P.,and Symington,L.S.(2009).DNA end resection:many nucleases make light work.DNA Repair(Amst.)8,
25、983995.Moynahan,M.E.,and Jasin,M.(2010).Mitotic homologous recombination maintains genomic stability and suppresses tumorigenesis.Nat.Rev.Mol.Cell Biol.11,196207.Prakash,R.,Satory,D.,Dray,E.,Papusha,A.,Scheller,J.,Kramer,W.,Krejci,L.,Klein,H.,Haber,J.E.,Sung,P.,et al.(2009).Yeast Mph1 helicase disso
26、ciates Rad51-made D-loops:implications for crossover control in mitotic recombination.Genes Dev.23,6779.San Filippo,J.,Sung,P.,and Klein,H.(2008).Mechanism of eukaryotic homologous recombination.Annu.Rev.Biochem.77,229257.Svendsen,J.M.,and Harper,J.W.(2010).GEN1/Yen1 and the SLX4 complex:Solutions to the problem of Holliday junction resolution.Genes Dev.24,521536.Wu,L.,and Hickson,I.D.(2003).The Blooms syndrome helicase suppresses crossing over during homologous recombination.Nature 426,870874.
