1、Journal of Visualized ECopyright 2011 Journal of Visualized ExperimentsPage 1 of 1Materials List for:Protein Membrane Overlay Assay:A Protocol to Test Interaction BetweenSoluble and Insoluble Proteins in vitroShoko Ueki1,Benot Lacroix1,Vitaly Citovsky11Department of Biochemistry and Cell Biology,Sta
2、te University of New YorkCorrespondence to:Shoko Ueki at suekims.cc.sunysb.eduURL:http:/ NumberCommentsProtein assay kitBio-Rad500-0001Proteinase inhibitor cocktailSigma-AldrichS8820Mini-PROTEAN systemBio-Rad165-8000Semi-dry western blotting SDelectrotransfer systemBio-Rad170-3940Anti-rabbit IgG antibody conjugatedwith horse radish peroxidaseGenScriptA00098Anti-GST rabbit polyclonal antibody GenScriptA00097Anti-strepIIGenScriptA00626BioTrace,NT nitrocellulose transfermembranePall Corporation27377-000Immobilon westernchemiluminescent HRP substrateEMD MilliporeWBKL S0 050
