1、Journal of Visualized ECopyright 2011 Journal of Visualized ExperimentsPage 1 of 1Materials List for:Extraction of High Molecular Weight DNA from Microbial MatsBenjamin S.Bey1,Erin B.Fichot1,R.Sean Norman11Department of Environmental Health Sciences,Arnold School of Public Health,University of South
2、 CarolinaCorrespondence to:R.Sean Norman at rsnormanmailbox.sc.eduURL:http:/ NumberComments-mercaptthanolSigma-AldrichM3148Polyethylene glycol 8000Promega Corp.V301120%in 1.2 M NaClPotassium acetateFisher ScientificFisher ScientificQuant-iT dsDNA Assay kitInvitrogenQ33130RNaseEpicentre Biotechnologi
3、esMRNA092Sodium ChlorideVWRBDH8014Appropriate conc.Sodium Dodecyl SulfateFisher Scientific03-500-50910%in watersodium hexametaphosphateEMD MilliporeSX0583-32%in waterTBEFisher ScientificBP1333-1CHEF Mapper XA SystemBio-Rad170-3670NanoDrop 1000 spectrophotometer Thermo Fisher Scientific,Inc.ND-1000VortexerScientific Industries Inc.Ultraviolet CrosslinkerUVP Inc.Waring blenderWaring LaboratoryLB10S
