1、SDS-PAGE分离胶配方表各种组份名称各种凝胶体积所对应的各种组份的取样量5ml10 ml15 ml20 ml25 ml30 ml40ml50 ml6%GelH2O2.65.37.910.613.215.921.226.530%Acrylamide1.02.03.04.05.06.08.010.01.5 M Tris-HCI(pH8.8)1.32.53.85.06.310.012.510%SDS0.050.10.150.20.250.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.0
2、48%GelH2O2.34.66.99.311.513.918.523.230%Acrylamide1.32.74.05.36.78.010.713.31.5 M Tris-HCI(pH8.8)1.32.53.85.06.37.51012.510%SDS0.050.10.150.250.30.510%过硫酸铵0.050.10.1500.2503840.5TEMED0.0030.0060.009.0120.0150.0180.0240.0310%GelH2O1.94.05.97.99.911.915.919.830%Acrylamide1.73.35.06.78.310.013.316.71.5
3、 M Tris-HCI(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0212%GelH2O1.6.4.96.68.29.913.216.530%Acrylamide2.006.08.010.012.016.020.01.5 M Tris-HCI(pH8.8)1.33.85.06.31012.510%SDS0.050.10.150.20.2530.510%过硫酸铵
4、0.050.10.150.20.250.3840.5TEMED0.0020.0040.0060.0080.010.0120.0160.0215%GelH2O1.12.33.44.65.76.99.211.530%Acrylamide5.07.510.012.515.020.025.01.5 M Tris-HCI(pH8.8)2.53.86.3710.012.510%SDS0.050.10.15020.25030.40.510%过硫酸铵0.050.10.150.20.250.0.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02TaKaRa BIOTECHNOLOGY CATALOG ONLINEhttp:/
