1、Designation:E124712(Reapproved 2017)Standard Practice forDetecting Fluorescence in Object-Color Specimens bySpectrophotometry1This standard is issued under the fixed designation E1247;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,t
2、he year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice provides spectrophotometric methods fordetecting the presence of fluorescence in object-color spec
3、i-mens.NOTE1Since the addition of fluorescing agents(colorants,whiteningagents,etc.)is often intentional by the manufacturer of a material,information on the presence or absence of fluorescent properties in aspecimen may often be obtained from the maker of the material.1.2 This practice requires the
4、 use of a spectrophotometerthat both irradiates the specimen over the wavelength rangefrom 340 to 700 nm and allows the spectral distribution ofillumination on the specimen to be altered as desired.1.3 Within the above limitations,this practice is general inscope rather than specific as to instrumen
5、t or material.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations pri
6、or to use.1.5 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBa
7、rriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D2244 Practice for Calculation of Color Tolerances andColor Differences from Instrumentally Measured ColorCoordinatesE284 Terminology of AppearanceE308 Practice for Computing the Colors of Objects by Usingthe CIE SystemE313 Prac
8、tice for Calculating Yellowness and WhitenessIndices from Instrumentally Measured Color CoordinatesE991 Practice for Color Measurement of Fluorescent Speci-mens Using the One-Monochromator MethodE1164 Practice for Obtaining Spectrometric Data for Object-Color EvaluationE1331 Test Method for Reflecta
9、nce Factor and Color bySpectrophotometry Using Hemispherical GeometryE1348 Test Method for Transmittance and Color by Spec-trophotometry Using Hemispherical GeometryE1349 Test Method for Reflectance Factor and Color bySpectrophotometry Using Bidirectional(45:0 or 0:45)GeometryE2152 Practice for Comp
10、uting the Colors of FluorescentObjects from Bispectral Photometric DataE2153 Practice for Obtaining Bispectral Photometric Datafor Evaluation of Fluorescent Color3.Terminology3.1 The definitions in Terminology E284,Practices E991,E2152,and E2153 are applicable to this practice.4.Significance and Use
11、4.1 Several standards,including Practices E991,E1164,andTest Methods E1331,E1348 and E1349,require either thepresence or absence of fluorescence exhibited by the specimenfor correct application.This practice provides spectrophoto-metric procedures for identifying the presence of fluorescencein mater
12、ials.4.2 This practice is applicable to all object-color specimens,whether opaque,translucent,or transparent,meeting the re-quirements for specimens in the appropriate standards listed in2.1.Translucent specimens should be measured by reflectance,with a standard non-fluorescent backing material,usua
13、lly butnot necessarily black,placed behind the specimen duringmeasurement.4.3 This practice requires the use of a spectrophotometer inwhich the spectral distribution of the illumination on thespecimen can be altered by the user in one of several ways.Themodification of the illumination can either be
14、 by the insertion1This practice is under the jurisdiction of ASTM Committee E12 on Color andAppearance and is the direct responsibility of Subcommittee E12.05 on Fluores-cence.Current edition approved Nov.1,2017.Published November 2017.Originallyapproved in 1988.Last previous edition approved in 201
15、2 as E1247 12.DOI:10.1520/E1247-12R17.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International
16、,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1 of optical filters between the illuminating source and thespecimen,without interfering with the detectio
