1、Designation:E152502(Reapproved 2014)Standard Guide forDesigning Biological Tests with Sediments1This standard is issued under the fixed designation E1525;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A num
2、ber in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 As the contamination of freshwater and saltwater eco-systems continues to be reduced through the implementation ofregulations governing both
3、point and non-point sourcedischarges,there is a growing emphasis and concern regardinghistorical inputs and their influence on water and sedimentquality.Many locations in urban areas exhibit significantsediment contamination,which poses a continual and long-term threat to the functional condition of
4、 benthic communitiesand other species inhabiting these areas(1).2Benthic commu-nities are an important component of many ecosystems andalterations of these communities may affect water-column andnonaquatic species.1.2 Biological tests with sediments are an efficient meansfor evaluating sediment cont
5、amination because they provideinformation complementary to chemical characterizations andecological surveys(2).Acute sediment toxicity tests can beused as screening tools in the early phase of an assessmenthierarchy that ultimately could include chemical measurementsor bioaccumulation and chronic to
6、xicity tests.Sediment testshave been applied in both saltwater and freshwater environ-ments(2-6).Sediment tests have been used for dredge materialpermitting,site ranking for remediation,recovery studiesfollowing management actions,and trend monitoring.A par-ticularly important application is for est
7、ablishing contaminant-specific effects and the processes controlling contaminantbioavailability(7).1.3 This guide is arranged as follows:SectionReferenced Documents2Terminology3Application4Summary of Guide5Significance and Use6Hazards7Sediment Test Types8Biological Responses9Test Organisms10Experime
8、ntal Design Considerations11Data Interpretation12Keywords131.4 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is therespo
9、nsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.For specific hazardstatements,see Section 7.2.Referenced Documents2.1 ASTM Standards:3D1129 Terminology Relating to WaterD4447 Guide fo
10、r Disposal of Laboratory Chemicals andSamplesE724 Guide for Conducting Static Acute Toxicity TestsStarting with Embryos of Four Species of SaltwaterBivalve MolluscsE729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating
11、to Biological Effects and Envi-ronmental FateE1023 Guide for Assessing the Hazard of a Material toAquatic Organisms and Their UsesE1367 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine In-vertebratesE1383 Guide for Conducting Sediment Toxicity Test
12、s withFreshwater Invertebrates(Withdrawn 1995)4E1391 Guide for Collection,Storage,Characterization,andManipulation of Sediments for Toxicological Testing andfor Selection of Samplers Used to Collect Benthic Inver-tebrates1This guide is under the jurisdiction ofASTM Committee E50 on EnvironmentalAsse
13、ssment,Risk Management and Corrective Action and is the direct responsibil-ity of Subcommittee E50.47 on Biological Effects and Environmental Fate.Current edition approved Oct.1,2014.Published May 2015.Originally approvedin 1993.Last previous edition approved in 2008 as E1525 02(2008).DOI:10.1520/E1
14、525-02R14.2The boldface numbers in parentheses refer to the list of references at the end ofthis standard.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards D
15、ocument Summary page onthe ASTM website.4The last approved version of this historical standard is referenced onwww.astm.org.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 E
16、1563 Guide for Conducting Static Acute Toxicity Testswith Echinoid EmbryosE1611 Guide for Conducting Sediment Toxicity Tests withPolychaetous AnnelidsE1676 Guide for Conducting Laboratory Soil Toxicity orBioaccumulation Tests with the Lumbricid EarthwormEisenia Fetida and the Enchytraeid Potworm EnchytraeusalbidusE1688 Guide for Determination of the Bioaccumulation ofSediment-Associated Contaminants by Benthic Inverte-bratesE1706 Test Method for Measuring the Toxicity of Sediment-Associated Cont
