1、Designation:E119103a(Reapproved 2014)Standard Guide forConducting Life-Cycle Toxicity Tests with Saltwater Mysids1This standard is issued under the fixed designation E1191;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of l
2、ast revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This guide describes procedures for obtaining laboratorydata concerning the adverse effects of a test material added todilut
3、ion water,but not to food,on certain species of saltwatermysids during continuous exposure from immediately afterbirth until after the beginning of reproduction using theflow-through technique.These procedures will probably beuseful for conducting life-cycle toxicity tests with other speciesof mysid
4、s,although modifications might be necessary.1.2 Other modifications of these procedures might be justi-fied by special needs or circumstances.Although using appro-priate procedures is more important than following prescribedprocedures,results of tests conducted using unusual proceduresare not likely
5、 to be comparable to results of many other tests.Comparison of results obtained using modified and unmodifiedversions of these procedures might provide useful informationon new concepts and procedures for conducting life-cycletoxicity tests with saltwater mysids.1.3 These procedures are applicable t
6、o all chemicals,eitherindividually or in formulations,commercial products,orknown mixtures,that can be measured accurately at thenecessaryconcentrationsinwater.Withappropriatemodifications,these procedures can be used to conduct tests ontemperature,dissolved oxygen,and pH and on such materialsas aqu
7、eous effluents(see also Guide E1192),leachates,oils,particulate matter,sediments,and surface waters.1.4 This guide is arranged as follows:SectionReferenced Documents2Terminology3Summary of Guide4Significance and Use5Hazards7Apparatus6Facilities6.1Construction Materials6.2Metering System6.3Test Chamb
8、ers6.4Cleaning6.5Acceptability6.6Dilution Water8Requirements8.1Source8.2Treatment8.3Characterization8.4Test Material9General9.1Stock Solution9.2Test Concentration(s)9.3Test Organisms10Species10.1Age10.2Source10.3Brood Stock10.4Food10.5Handling10.6Harvesting Young10.7Quality10.8Procedure11Experimenta
9、l Design11.1Dissolved Oxygen11.2Temperature11.3Beginning the Test11.4Feeding11.5Cleaning11.6Duration of Test11.7Biological Data11.8Other Measurements11.9Analytical Methodology12Acceptability of Test13Calculation14Documentation15Keywords16AppendixX1.Statistical Guidance1.5 This standard does not purp
10、ort to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.Specific hazardstatements are given in Section 7.
11、2.Referenced Documents2.1 ASTM Standards:2E729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-ians1This guide is under the jurisdiction ofASTM Committee E50 on EnvironmentalAssessment,Risk Management and CorrectiveActionand is the direct responsi
12、bilityof Subcommittee E50.47 on Biological Effects and Environmental Fate.Current edition approved Oct.1,2014.Published December 2014.Originallyapproved in 1987.Last previous edition approved in 2008 as E1191 03a(2008).DOI:10.1520/E1191-03AR14.2For referenced ASTM standards,visit the ASTM website,ww
13、w.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 E943 Termino
14、logy Relating to Biological Effects and Envi-ronmental FateE1023 Guide for Assessing the Hazard of a Material toAquatic Organisms and Their UsesE1192 Guide for Conducting Acute Toxicity Tests on Aque-ous Ambient Samples and Effluents with Fishes,Macroinvertebrates,and AmphibiansE1203 Practice for Us
15、ing Brine Shrimp Nauplii as Food forTest Animals in Aquatic Toxicology(Withdrawn 2013)3IEEE/ASTM SI 10 American National Standard for Use ofthe International System of Units(SI):The Modern MetricSystem3.Terminology3.1 The words“must,”“should,”“may,”“can,”and“might”have very specific meanings in this
16、 guide.3.1.1“Must”is used to express an absolute requirement,that is,to state that the test ought to be designed to satisfy thespecified condition,unless the purpose of the test requires adifferent design.“Must”is only used in connection with factorsthat directly relate to the acceptability of the test(see 13.1).3.1.2“Should”is used to state that the specified condition isrecommended and ought to be met if possible.Althoughviolation of one“should”is rarely a serious matter,violation ofseveral wi
