1、Designation:E129501(Reapproved 2013)Standard Guide forConducting Three-Brood,Renewal Toxicity Tests withCeriodaphnia dubia1This standard is issued under the fixed designation E1295;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the
2、year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This guide describes procedures for obtaining dataconcerning the adverse effects of an effluent or a test material
3、(added to dilution water,but not to food)on Ceriodaphniadubia Richard 1894,during continuous exposure throughout aportion of the organisms life.These procedures should also beuseful for conducting life cycle toxicity tests with otherCladocera(Guide E1193),although modifications will benecessary.1.2
4、These procedures are applicable to most chemicals,either individually or in formulations,commercial products,orknown mixtures,that can be measured accurately at thenecessary concentrations in water.With appropriate modifica-tions these procedures can be used to conduct tests ontemperature,dissolved
5、oxygen,pH,and on such materials asaqueous effluents(see also Guide E1192),leachates,oils,particulate matter,sediments(see also Guide E1383),andsurface waters.Renewal tests might not be applicable tomaterials that have high oxygen demand,are highly volatile,are rapidly biologically or chemically tran
6、sformed,or sorb totest chambers.If the concentration of dissolved oxygen fallsbelow 4 mg/Lor the concentration of test material decreases bymore than 20%in test solution(s)between renewals,morefrequent renewals might be necessary.1.3 Other modifications of these procedures might be justi-fied by spe
7、cial needs or circumstances.Results of tests con-ducted using unusual procedures are not likely to be compa-rable to results of many other tests.Comparisons of resultsobtained using modified and unmodified versions of theseprocedures might provide useful information on new conceptsand procedures for
8、 conducting three-brood toxicity tests withC.dubia.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of reg
9、ulatory limitations prior to use.Specific hazardstatements are given in Section 8.1.5 This guide is arranged as follows:SectionReferenced Documents2Terminology3Summary of Guide4Significance and Use5Apparatus6Facilities6.1Construction Materials6.2Test Chambers6.3Cleaning6.4Reagents and Materials7Haza
10、rds8Dilution Water9Requirements9.1Source9.2Treatment9.3Characterization9.4Test Material10General10.1Stock Solution10.2Effluent10.3Test Concentration(s)10.4Collection10.5Sample Containers10.6Preservation10.7Treatment10.8Test Organisms11Species11.1Age11.2Source11.3Brood Stock11.4Food11.5Handling11.6Qu
11、ality11.7Procedure12Demonstration of Feasibility12.1Experimental Design12.2Dissolved Oxygen12.3Temperature12.4Preparing Test Solutions12.5Conditioning Test Chambers12.6Beginning a Test12.7Renewing Test Solutions12.8Duration of Test12.9Biological Data12.10Other Measurements12.11Analytical Methodology
12、13Acceptability of Test14Calculation15Report161This guide is under the jurisdiction ofASTM Committee E50 on EnvironmentalAssessment,Risk Management and CorrectiveActionand is the direct responsibilityof Subcommittee E50.47 on Biological Effects and Environmental Fate.Current edition approved March 1
13、,2013.Published March 2013.Originallyapproved in 1989.Last previous edition approved in 2006 as E1295 01(2006).DOI:10.1520/E1295-01R13.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.Unite
14、d States1 AppendixesFoodAppendix X1Culture TechniquesAppendix X2Test ChambersAppendix X3Statistical GuidanceAppendix X42.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterD3978 Practice for Algal Growth Potential Testing withPseudokirchneriella subcapitataE729 Guide for Co
15、nducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating to Biological Effects and Envi-ronmental FateE1023 Guide for Assessing the Hazard of a Material toAquatic Organisms and Their UsesE1192 Guide for Conducting Acute Toxicity Tests on
16、Aque-ous Ambient Samples and Effluents with Fishes,Macroinvertebrates,and AmphibiansE1193 Guide for Conducting Daphnia magna Life-CycleToxicity TestsE1383 Guide for Conducting Sediment Toxicity Tests withFreshwater Invertebrates(Withdrawn 1995)3E1706 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater InvertebratesIEEE/ASTM SI 10 American National Standard for Use ofthe International System of Units(SI):The Modern MetricSystem3.Terminology3.1 The words“must
