1、Designation:D842122Standard Test Method forDetermination of Per-and Polyfluoroalkyl Substances(PFAS)in Aqueous Matrices by Co-solvation followed byLiquid Chromatography Tandem Mass Spectrometry(LC/MS/MS)1This standard is issued under the fixed designation D8421;the number immediately following the d
2、esignation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the determ
3、ination of per-andpolyfluoroalkyl substances(PFASs)in aqueous matrices usingliquid chromatography(LC)and detection with tandem massspectrometry(MS/MS).These analytes are co-solvated by a1+1 ratio of sample and methanol then qualitatively andquantitatively determined by this test method.Quantitation
4、isby selected reaction monitoring(SRM)or sometimes referredto as multiple reaction monitoring(MRM).1.2 The method detection limit(MDL)(see Note 1)andreporting range(see Note 2)for the target analytes are listed inTable 1.The target concentration for the reporting limit for thistest method is an inte
5、ger value that is calculated from theconcentration from the lowest standard from the final volumeof the prepared sample.This value may be lower than thecalculated MDL due to sporadic PFAS hits due to PFAScontamination in consumables/collection tools used duringsample collection and preparation.All s
6、amples should be takenat a minimal as duplicates in order to compare the precisionbetween the two prepared samples to help ensure theconcentration/positive result is reliable.NOTE1The MDL is determined following the Code of FederalRegulations(CFR),40 CFR Part 136,Appendix B utilizing dilution andfil
7、tration.A detailed process determining the MDL is explained in thereference and is beyond the scope of this test method.NOTE2Injection volume variations,and sensitivity of the instrumentused will change the reporting limit and ranges.1.2.1 Recognizing continual advancements in the sensitivityof inst
8、rumentation,advancements in column chromatographyand other processes not recognized here,the reporting limitmay be lowered assuming the minimum performance require-ments of this test method at the lower concentrations are met.1.2.2 Depending on data usage,you may modify this testmethod but limit to
9、modifications that improve performancewhile still meeting or exceeding the method quality acceptancecriteria.Modifications to the solvents,ratio of solvent tosample,or shortening the chromatographic run simply to savetime are not allowed.Use Practice E2935 or similar statisticaltests to confirm that
10、 modifications produce equivalent resultson non-interfering samples.In addition,use Guide E2857 orequivalent statistics to re-validate the modified test.1.2.3 Analyte detections between the method detection limitand the reporting limit are estimated concentrations.Thereporting limit is based upon th
11、e concentration of the Level 1calibration standard as shown in Table 5.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It
12、is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accor-dance with internationally recognized principles on sta
13、ndard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD1193 Specifi
14、cation for Reagent WaterD2777 Practice for Determination of Precision and Bias ofApplicable Test Methods of Committee D19 on Water1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.06 on Methods forAnalysis forOrganic Substa
15、nces in Water.Current edition approved May 1,2022.Published June 2022.Originallyapproved in 2021.Last previous edition approved in 2021 as D8421 21.DOI:10.1520/D8421-22.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Bo
16、ok of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barrier
