1、Designation:D558909(Reapproved 2013)Standard Test Method forDetermining the Resistance of Paint Films and RelatedCoatings to Algal Defacement1This standard is issued under the fixed designation D5589;the number immediately following the designation indicates the year oforiginal adoption or,in the ca
2、se of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers an accelerated method fordetermining the relative resistance of a paint
3、or coating film toalgal growth.NOTE1It is hoped that a ranking of relative performance would besimilar to that ranked from outdoor exposures.However,this test methodshould not be used as a replacement for exterior exposure since manyother factors,only a few of which are listed will affect those resu
4、lts.1.2 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish app
5、ro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D822 Practice for Filtered Open-Flame Carbon-Arc Expo-sures of Paint and Related CoatingsD4141 Practice for Conducting Black Box and Solar Con-centr
6、ating Exposures of CoatingsD4587 Practice for Fluorescent UV-Condensation Expo-sures of Paint and Related CoatingsD5031 Practice for Enclosed Carbon-Arc Exposure Tests ofPaint and Related CoatingsD6695 Practice for Xenon-Arc Exposures of Paint andRelated Coatings3.Summary of Test Method3.1 This test
7、 method outlines a procedure to(1)prepare asuitable specimen for testing,(2)inoculate the specimen witha mixture of the proper algal species,(3)expose the inoculatedsamples under the appropriate conditions for growth,and(4)provide a schedule and guidelines for visual growth ratings.This test method
8、is not designed to include all the necessaryprocedures to maintain the proper microbiological techniquesrequired to provide the most accurate results.4.Significance and Use4.1 Defacement of paint and coating films by algal growth isa common phenomenon under certain conditions.It is gener-ally known
9、that differences in the environment,lighting,temperature,substrate,and other factors in addition to thecoating composition affect the susceptibility of a given paintedsurface.This test method attempts to provide a means tocomparatively evaluate different coating formulations for theirrelative perfor
10、mance under a given set of conditions.It doesnot imply that a coating that resists growth under theseconditions will necessarily resist growth in the actual applica-tion(see Note 1).4.2 Familiarity with microbiological techniques is required.This test method should not be used by persons without at
11、leastbasic microbiological training.5.Apparatus and Materials5.1 Balance,capable of weighing to 0.10 g.5.2 Incubator,or other device capable of maintaining aconstant temperature between 25 6 2C,relative humidity of85%,and having a constant fluorescent light source.5.3 Refrigerator.5.4 Petri Dishes,1
12、00 by 15 mm(3.9 by 0.6 in.).5.5 Autoclave.5.6 Paint Brush,coarse bristle,12 to 19 mm(12to34in.).5.7 Test Substrate,filter paper,either regular paper or glassfiber,4.2 cm(1.65 in.)in diameter,or drawdown paper(unlaquered chart paper)21.6 by 28.0 cm(8.5 by 11 in.),cutinto ten 21.6 by 2.8-cm(8.5 by 1.1
13、-in.)strips may be used.5.8 Tissue Grinder.5.9 Atomizer or Chromatography Sprayer.1This test method is under the jurisdiction of ASTM Committee D01 on Paintand Related Coatings,Materials,and Applications and is the direct responsibility ofSubcommittee D01.28 on Biodeterioration.Current edition appro
14、ved Oct.1,2013.Published October 2013.Originallyapproved in 1994.Last previous edition approved in 2009 as D5589 09.DOI:10.1520/D5589-09R13.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume in
15、formation,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.10 Sterile Glass Rods,Forceps,250-mL Glass ErlenmeyerFlask,and other routine microbiological equipment.5.11 Allens
16、 Medium3or Bolds Basal Medium4ingredients(see 6.3).5.12 Distilled Water.6.Reagents and Materials6.1 Purity of ReagentsReagent grade chemicals should beused in all tests.Unless otherwise indicated,it is intended thatall reagents should conform to the specifications of theCommittee on Analytical Reagents of the American ChemicalSociety,where such specifications are available.5Other gradesmay be used,provided they are first ascertained to be ofsufficiently high purity to permit use without decreasi
