1、Designation:C137910Standard Test Method forAnalysis of Urine for Uranium-235 and Uranium-238Isotopes by Inductively Coupled Plasma-MassSpectrometry1This standard is issued under the fixed designation C1379;the number immediately following the designation indicates the year oforiginal adoption or,in
2、the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the determination of the con-centration of uranium-235 and uranium
3、-238 in urine usingInductively Coupled Plasma-Mass Spectrometry.This testmethod can be used to support uranium facility bioassayprograms.1.2 This method detection limits for235U and238U are 6ng/L.To meet the requirements ofANSI N13.30,the minimumdetectable activity(MDA)of each radionuclide measured
4、mustbe at least 0.1 pCi/L(0.0037 Bq/L).The MDA translates to 47ng/L for235U and 300 ng/L for238U.Uranium 234 cannot bedetermined at the MDAwith this test method because of its lowmass concentration level equivalent to 0.1 pCi/L.1.3 The digestion and anion separation of urine may not benecessary when
5、 uranium concentrations of more than 100 ng/Lare present.1.4 UnitsThe values stated in picoCurie per liter units areto be regarded as standard.The values given in parentheses aremathematical conversions to SI units that are provided forinformation only and are not considered standard.1.5 This standa
6、rd does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.NOTE1Warning:The ICP-MS is a sour
7、ce of intense ultravioletradiation from the radio frequency induced plasma.Protection from radiofrequency radiation and UV radiation is provided by the instrument undernormal operation.2.Referenced Documents2.1 ASTM Standards:2C859 Terminology Relating to Nuclear MaterialsC1310 Test Method for Deter
8、mining Radionuclides in Soilsby Inductively Coupled Plasma-Mass Spectrometry UsingFlow Injection Preconcentration(Withdrawn 2011)3C1345 Test Method for Analysis of Total and IsotopicUranium and Total Thorium in Soils by InductivelyCoupled Plasma-Mass SpectrometryD1193 Specification for Reagent Water
9、2.2 Other Documents:ANSI N13.30 Radiological Measurement QualityDOE Order 5480.11 Radiological Measurements Quality3.Terminology3.1 For terms in this document,see Terminology C859.3.2 Definitions:3.2.1 isobar,nany atom that has the same atomic massnumber as another atom but a different atomic number
10、.3.3 Acronyms:3.3.1 AMU,natomic mass unit3.3.2 CB,ncalibration blank3.3.3 COC,nchain of custody3.3.4 CVS,ncalibration verification standard3.3.5 ICS,ninstrument check standard3.3.6 IDL,ninstrument detection limit3.3.7 LCS,nlaboratory control sample3.3.8 MDA,nminimum detectable activity1This test met
11、hod is under the jurisdiction ofASTM Committee C26 on NuclearFuel Cycle and is the direct responsibility of Subcommittee C26.05 on Methods ofTest.Current edition approved Jan.1,2010.Published February 2010.Originallyapproved in 1997.Last previous edition approved in 2004 as C1379 04.DOI:10.1520/C137
12、9-10.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandardsvolume information,refer to the standards Document Summary page onthe ASTM website3The last approved version of this historical standard is refer
13、enced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.3.9 m/e,nmass/charge ratio3.3.10 RMDA,nrequired minimum detectable activity3.3.11%RDS,npercent relative standard deviation(1Standard Deviation/Mean)*1004.Summary of Te
14、st Method4.1 A urine sample is digested and wet oxidized withconcentrated nitric and hydrochloric acids to solubilize ura-nium and to destroy the organic matter.Uranium is selectivelyseparated from the chloride salts by an anion exchange resinand is eluted with dilute nitric acid.The235U and238U iso
15、topesare determined by ICP-MS.5.Significance and Use5.1 DOE Order 5480.11 and ANSI N13.30 require thatinternal dose assessments be made as part of the bioassayprogram for nuclear facility workers.For indirect bioassay ofuranium workers,the uranium isotopes must be measuredalong with the total uraniu
16、m in urine samples.The RMDA foreach uranium isotope is 0.1 pCi/L.5.2 This method is applicable for measuring235U and238Uat the RMDA.Because of extremely low mass concentration(because of the high specific activity),234U cannot be mea-sured without additional sample preconcentration.NOTE2Column chromatography separations and concentration of234U using manual or flow-injection preconcentration followed byICP-MS isotopic determination are described in Test Methods C1310 andC1345.These methods focus
