1、Designation:E227514Standard Practice forEvaluating Water-Miscible Metalworking Fluid Bioresistanceand Antimicrobial Pesticide Performance1This standard is issued under the fixed designation E2275;the number immediately following the designation indicates the year oforiginal adoption or,in the case o
2、f revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice addresses the evaluation of the relativeinherent bioresistance of water-miscible metal
3、working fluids,the bioresistance attributable to augmentation with antimicro-bial pesticides or both.It replaces Methods D3946 and E686.1.2 In this practice relative bioresistance is determined bychallenging metalworking fluids with a biological inoculumthat may either be characterized(comprised of
4、one or moreknown biological cultures)or uncharacterized(comprised ofbiologically contaminated metalworking fluid or one or moreunidentified isolates from deteriorated metalworking fluid).Challenged fluid bioresistance is defined in terms of resistanceto biomass increase,viable cell recovery increase
5、,chemicalproperty change,physical property change or some combina-tion thereof.1.3 This practice is applicable to antimicrobial agents thatare incorporated into either the metalworking fluid concentrateor end-use dilution.It is also applicable to metalworking fluidsthat are formulated using non-micr
6、obicidal,inherently biore-sistant components.1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of th
7、e user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD888 Test Methods for Dissolved Oxygen in WaterD1067 Test Methods for Aci
8、dity or Alkalinity of WaterD1193 Specification for Reagent WaterD3342 Test Method for Dispersion Stability of New(Un-used)Rolling Oil Dispersions in WaterD3519 Test Method for Foam in Aqueous Media(BlenderTest)(Withdrawn 2013)3D3601 Test Method for Foam In Aqueous Media(BottleTest)(Withdrawn 2013)3D
9、4627 Test Method for Iron Chip Corrosion for WaterMis-cible Metalworking FluidsD5465 Practice for Determining Microbial Colony Countsfrom Waters Analyzed by Plating MethodsE70 Test Method for pH of Aqueous Solutions With theGlass ElectrodeE1326 Guide for Evaluating Non-culture MicrobiologicalTests U
10、sed for Enumerating BacteriaE2169 Practice for Selecting Antimicrobial Pesticides forUse in Water-Miscible Metalworking FluidsE2523 Terminology for Metalworking Fluids and Opera-tionsE2563 Practice for Enumeration of Non-Tuberculosis Myco-bacteria in Aqueous Metalworking Fluids by Plate CountMethodE
11、2564 Practice for Enumeration of Mycobacteria in Metal-working Fluids by Direct Microscopic Counting(DMC)MethodE2657 Test Method for Determination of Endotoxin Con-centrations in Water-Miscible Metalworking FluidsE2694 Test Method for Measurement of Adenosine Triphos-phate in Water-Miscible Metalwor
12、king FluidsE2756 Terminology Relating to Antimicrobial and AntiviralAgentsE2889 Practice for Control of Respiratory Hazards in theMetal Removal Fluid Environment2.2 Other Standards:AOAC 960.9 Germicidal and Detergent Sanitizing ActionDisinfectants41This practice is under the jurisdiction of ASTM Com
13、mittee E35 on Pesticides,Antimicrobials,and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.15 on Antimicrobial Agents.Current edition approved Aug.1,2014.Published September 2014.Originallyapproved in 2003.Last previous edition approved in 2013 as E2275 13.DOI:10.1520
14、/E2275-14.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard i
15、s referenced onwww.astm.org.4AOAC International Methods of Analysis,AOAC International,Gaithersburg,MD.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 9215A.6a Heterotrophic Plate Count Media,Plate CountAgar59216 Direct Total Microbial Co
16、unt5Microbiological Test 62.3 Government Standard:40 CFR 156 Labeling Requirements for Pesticides and De-vices3.Terminology3.1 For definitions of terms used in this guide refer toTerminologies D1129,E2523,and E2756.3.2 Definitions:3.2.1 active ingredient,nthe chemical component or com-ponents of an antimicrobial pesticide that provides its micro-bicidal performance.3.2.2 antimicrobial pesticide,nchemical additive regis-tered under 40 CFR 152,for use to inhibit growth,proliferationor both of micr
