1、Designation:E227409Standard Test Method forEvaluation of Laundry Sanitizers and Disinfectants1This standard is issued under the fixed designation E2274;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A numbe
2、r in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in top-loadingautomatic clothes washing op
3、erations.This test method isdesigned predominantly to provide testing with representativevegetative bacteria but can also be designed to accommodatethe testing of fungi and viruses.NOTE1This test method does not evaluate sanitizing/disinfectantlaundry detergent/additives for use in front-loading,low
4、 water volumeautomatic clothes washing operations.1.2 Knowledge of microbiological techniques is requiredfor these procedures.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.NOTE2In this method,metric units are used for all a
5、pplications,except for distance in which case inches are used.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bi
6、lity of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE177 Practice for Use of the Terms Precision and Bias inASTM Test MethodsE691 Practice for Conducting an Interlaboratory Study toDetermine the Precision of a Test MethodE1054 Te
7、st Methods for Evaluation of Inactivators of Anti-microbial Agents2.2 Other Documents:AATCC Test Method 70-1997 Water Repellency;TumbleJar Dynamic Absorption Test3DIS/TSS13 LaundryAdditivesDisinfectionandSanitization,U.S.Environmental Protection Agency,Of-fice of Pesticide Programs,April 198043.Term
8、inology3.1 Definitions:3.1.1 active antimicrobial ingredienta substance added toa formulation intended specifically for the inhibition or inac-tivation of microorganisms.3.1.2 antimicrobial agent(s)an active ingredient designedto suppress the growth or action of microorganisms.3.1.3 carrier count co
9、ntrolprocedure used to determinethe initial number of microorganisms on a fabric carrierfollowing the inoculation and drying procedure.3.1.4 diluentsterile deionized water,sterile distilled wateror sterile synthetic AOAC hard water that may be used toprepare the active test formulation,vehicle contr
10、ol or productcontrol for use in the test procedure.3.1.5 diluted product solutiontest formulation,vehiclecontrol,or product control diluted to use concentration.3.1.6 neutralizationa process that results in quenching theantimicrobial activity of a test formulation.This may beachieved by dilution of
11、the test formulation(s)to reduce theconcentration of the antimicrobials,or through the use ofchemical agents,called neutralizers,to suppress antibacterialactivity.3.1.7 numberscontrolinassessingsanitizerlevelperformance,procedure used to determine the number ofmicroorganisms remaining on the fabric
12、carriers and in thewash water following the test procedure in the presence of thediluent.This may also be performed using diluent or phosphatebuffer dilution water with surfactant.1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Ag
13、ents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved Nov.1,2009.Published December 2009.Originallyapproved in 2003.Last previous edition approved in 2003 as E2247 03.DOI:10.1520/E2274-09.2For referenced ASTM standards,visit the ASTM website,www
14、.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from American Association of Textile Chemists and Colorists(AATCC),P.O.Box 12215,Research Triangle Park,NC 27709
15、,http:/www.aatcc.org.4Available from United States Environmental Protection Agency(EPA),ArielRios Bldg.,1200 Pennsylvania Ave.,NW,Washington,DC 20460,http:/www.epa.gov.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.1.8 product controla
16、 formulation with or without anactive ingredient(s)used for comparison to the test formula-tion.3.1.9 test formulationa formulation containing an antimi-crobial agent(s).3.1.10 vehicle controlthe test formulation without theactive ingredient(s)used for comparison to the test formula-tion.3.1.11 wash waterthe liquid contained in the exposurechamber previously exposed to either uninoculated fabric orfabric inoculated with the challenge microorganism.4.Summary of Test Method4.1 Under simulated laun
