1、Designation:D401215Standard Test Method forAdenosine Triphosphate(ATP)Content of Microorganismsin Water1This standard is issued under the fixed designation D4012;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revisi
2、on.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers a protocol for capturing,ex-tracting and quantifying the cellular adenosine triphosphate(cATP)content associat
3、ed with microorganisms normallyfound in laboratory cultures,waters,wastewaters,and inplankton and periphyton samples from waters.1.2 The ATP is measured using a bioluminescence enzymeassay,whereby light is generated in amounts proportional tothe concentration of ATP in the samples.The light is produ
4、cedand measured quantitatively as relative light units(RLU)which are converted by comparison with an ATP standard andcomputation to pg ATP/mL.1.3 This method does not remove all known chemicalinterferences,known to either luminesce in the 530 6 20 nmrange,or to quench light emitted in that range.It
5、should not beused to determine ATP concentrations in samples with dis-solved organic compounds,heavy metals or 10 000 ppm totaldissolved solids.Alternative methods have been developed fordetermining ATP concentrations in fluids samples likely tocontain such interferences(Test Methods D7687 and E2694
6、).1.4 Knowledge of the concentration ofATPcan be related toviable biomass or metabolic activity of microorganisms(Ap-pendix X1).1.5 This test method offers a high degree of sensitivity,rapidity,accuracy,and reproducibility.1.6 The analyst should be aware that the precision statementpertains only to
7、determinations in reagent water and notnecessarily in the matrix being tested.1.7 This test method is equally suitable for use in thelaboratory or field.1.8 The method normally detects cATPconcentrations in therange of 0.1 pg cATP/mL(1.0Log10pg cATP/mL)to 4 000000 pg cATP/mL(6.6 Log10pg cATP/mL)in 5
8、0 mL watersamples.1.9 Providing interferences can be overcome,biolumines-cence is a reliable and proven method for qualifying andquantifying ATP,although the method does not differentiatebetween ATP from different sources,for example,from differ-ent types of microorganisms,such as bacteria,fungi,alg
9、ae andprotozoa.1.10 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.11 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to e
10、stablish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD1601 Test Method for Dilute Solution Viscosity of Ethyl-ene Pol
11、ymersD4175 Terminology Relating to Petroleum,PetroleumProducts,and LubricantsD5847 Practice for Writing Quality Control Specificationsfor Standard Test Methods for Water AnalysisD6161 Terminology Used for Microfiltration,Ultrafiltration,Nanofiltration and Reverse Osmosis Membrane ProcessesD6300 Prac
12、tice for Determination of Precision and BiasData for Use in Test Methods for Petroleum Products andLubricantsD7687 Test Method for Measurement of Cellular AdenosineTriphosphate in Fuel,Fuel/Water Mixtures,and Fuel-Associated Water with Sample Concentration by FiltrationE2694 Test Method for Measurem
13、ent of Adenosine Triphos-phate in Water-Miscible Metalworking FluidsF1671 Test Method for Resistance of Materials Used in1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved Ju
14、ly 1,2015.Published September 2015.Originallyapproved in 1981.Last previous edition approved in 2009 as D4012 81(2009).DOI:10.1520/D4012-15.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume in
15、formation,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 Protective Clothing to Penetration by Blood-Borne Patho-gens Using Phi-X174 Bacteriophage Penetration as a TestSyste
16、m3.Terminology3.1 Definitions:3.1.1 For definitions of terms used in this standard,refer toTerminologies D1129 and D4175.3.1.2 adenosine monophosphate(AMP),nmoleculeformed by the removal of two molecules of phosphate(onepyrophosphate molecule)from ATP.3.1.3 adenosine triphosphate(ATP),nmolecule com-prised of a purine and three phosphate groups that serves as theprimary energy transport molecule in all biological cells.3.1.4 aseptic,adjsterile,free from viable microbial con-tamination.3.1.5 backg
